中国现代医学杂志2007，Vol.17Issue(14)：1665-1669,1673,6.
LC-MS法快速测定大鼠血浆五味子乙素及其药代动力学分析
Rapid determination of Schisandrin B by liquid chromatography mass spectrometry and analysis of plasma pharmaeokinetics in rat
陈玮莹 1李慧 2罗文鸿 2蔡绍先 3温博贵4
作者信息
- 1. 汕头大学医学院,生物化学教研室,广东,汕头,515041
- 2. 汕头大学医学院,中心实验室,广东,汕头,515041
- 3. 汕头大学医学院,人体解剖教研室,广东,汕头,515041
- 4. 汕头大学医学院,病理学教研室,广东,汕头,515041
- 折叠
摘要
Abstract
[Objective] To develop a method for determination of Schisandrin B by LC-MS and to observe the pharmacological action of Schisandrin B in Wistar rat. [Methods] The efficacious components was extracted from the fruit of Schisandra chinensis (Turcz.) Baill. The extract was orally administrated to Wistar rat. Blood samples were obtained at different time points after the extract was administrated. The plasma concentration of Schisandrin B was analyzed by LC-MS, and the plasma concentration-time data of Schisandrin B was also analyzed. The parameters of pharmacokinetics of Schisandrin B in the rats were calculated with 3p97 pharmacokinetics program. [Results] The method was simple and rapid for determination of Schisandrin B in different samples. The retention time of the Schisandrin B was 2.96 min. The maximum mean plasma level of Schisandrin B was 1.01 μg/mL which was reached at 5.65 hr after the oral administration. The mean value of area under concentration-time curve (AUC) was be successfully applied to analysis of Schisandrin B in different samples and to investigating the pharmacokinetics action of Schisandrin B in experimental animals.关键词
大鼠/五味子乙素/LC-MS/药代动力学Key words
rat/Schisandrin B/LC-MS/pharmacokinetics分类
医药卫生引用本文复制引用
陈玮莹,李慧,罗文鸿,蔡绍先,温博贵..LC-MS法快速测定大鼠血浆五味子乙素及其药代动力学分析[J].中国现代医学杂志,2007,17(14):1665-1669,1673,6.
