中国临床康复2003，Vol.7Issue(1)：170-171,2.
构建MYC反应元件修饰hTERT核心启动子引导荧光素酶表达的腺病毒载体
Construction of adenoviral vector for luciferase driven by hTERT core promoter modified with MYC-responsive elements
杨旻 1王臻 1李立文 1苏明权 2于文彬2
作者信息
- 1. Institute of Orthopaedics,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,Shaanxi Province,China
- 2. Laboratory of Clinical Molecular biology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,Shaanxi Province,China
- 折叠
摘要
Abstract
AIM:To construct adenoviral vectors for luciferase driven by human telomerase reverse transcriptase(hTERT) core promoter with multimer of MYC responsive elements.METHODS:Multimer of MYC responsive elements was cloned into the upstream site of hTERT core promoter and the modified hTERT promoter and luciferase were cloned into the plasmid pDC316 to construct shuttle plasmids which cotransfected HEK 293 cells with rescue plasmid pBHGlox(delta)E1,3Cre to achieve recombinant adenoviral vectors.The cytopathic effects and PCR using primers specific for luciferase were used to identify the recombinant adenoviral vectors.RESULTS:Adenoviral vectors with luciferase driven by hTERT core promoter with none or positive and negative six copies of MYC responsive elements were constructed and amplified.The titer of the adenovirus were 3.5× 106 pfu/ml,2.5× 106 pfu/ml and 1.5× 106 pfu/ml respectively determined by plaque assay.CONCLUSION:The further research on transciriptional targeting in osteosarcoma gene therapy can be done using adenoviral vectors with luciferase driven by hTERT promoter with MYC responsive elements.关键词
adenoviruses,human/telomere/enzymology gones/neoplasmsKey words
adenoviruses,human/telomere/enzymology gones/neoplasms分类
医药卫生引用本文复制引用
杨旻,王臻,李立文,苏明权,于文彬..构建MYC反应元件修饰hTERT核心启动子引导荧光素酶表达的腺病毒载体[J].中国临床康复,2003,7(1):170-171,2.
