肿瘤防治研究2001，Vol.28Issue(3)：170-172,3.
阿霉素加热化疗对抗人肝癌耐药细胞多药耐药性的实验研究
The Relation Between nm23,LN R and Lymph Node Metastasis of Cholangiocarcinomas
摘要
Abstract
Objective To comparatively observe the effects ofthermo-chemotherapy of ADM on human multidrug-resistant hepatocellular carcinoma cell line (7721/Adm) to sensitivity and intracellular concentration of ADM.Methods By means of water bath and MTT assay,the sensitivity of 7721/Adm to ADM was observed,and the effects of hyperthermia on intracellular concentration of ADM of 7721/Adm was detected by flow cytometry.Results(1) Cell line survive rates of 7721/Adm were 70.2%、40.8% after chemotherapy and thermo-chemotherapy of ADM on in 30min,and that of 7721/Adm were 60.2%、37.4% in 60min.(2) intracellular concentration of ADM of 7721/Adm were 41.3%、92.0% after chemotherapy and thermo-chemotherapy of ADM on in 30min,detected by flow cytometry.Conclusion Heat treatment can apparently confronte multidrug-resistant of 7721/Adm and increase the sensitivity of 7721/Adm to ADM.,and the resulet indicate that increasing the sensitivity can be related to intracellular concentration of ADM.关键词
阿霉素(ADM)/热化疗/人肝癌细胞-7721/多药耐药分类
医药卫生引用本文复制引用
张洪新,刘燕,王执民,郭卫平,王义清,倪代慧,曹伟,樊爱琳,李文献,关彦..阿霉素加热化疗对抗人肝癌耐药细胞多药耐药性的实验研究[J].肿瘤防治研究,2001,28(3):170-172,3.基金项目
第四军医大学创新工程基金资助项目(CX99A016) （CX99A016）
