北京大学学报（医学版）2001，Vol.33Issue(2)：170-172,3.
白细胞介素-6与其受体及急性期蛋白反应因子在多发性骨髓瘤病人外周血中的表达
The expression and role of IL-6, IL-6R and acute phase reaction factor in peripheral blood mononuclear cells of multiple myeloma
黄晓军 1段萱 1黎燕 2林红 2付剑峰 1陆道培1
作者信息
- 1. 北京大学人民医院血液病研究所，北京 100044
- 2. 中国人民解放军军事医学科学院
- 折叠
摘要
Abstract
Objective： To study the expression and role of IL-6, IL-6 receptor(IL-6R) and acute phase reaction factor (APRF) in PMNC of multiple myeloma(MM) patients. Methods:The bioactivity of IL-6 and the level of sIL-6R in sera of MM patients were measured using IL-6 dependent cell line 7TD1 and ELISA respectively. The APRF activity of PMNC DNA binding protein was observed by gel blocking electrophoresis (EMSA). Results:The bioactivity of IL-6 and the level of sIL-6R in MM sera were significantly higher than those of the control and the level was coordinated with that of the tumor. The results of PMNC DNA binding protein EMSA showed that PMNC of MM patients expressed APRF after stimulation of IL-6,whereas there was no activity of APRF in the normal PMNC. Conclusion:In MM,some regulation machines of IL-6 signal transduction pathways changed (eg.protein kinase), resulting in the activation of transcription of acute phase reaction element(APRE), APRF may have a role in pathogenesis of MM.关键词
白细胞介素-6/血液/受体,白细胞介素/血液/多发性骨髓瘤/血液/急性期蛋白反应因子分类
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黄晓军,段萱,黎燕,林红,付剑峰,陆道培..白细胞介素-6与其受体及急性期蛋白反应因子在多发性骨髓瘤病人外周血中的表达[J].北京大学学报（医学版）,2001,33(2):170-172,3.
