中华医学杂志（英文版）2008，Vol.121Issue(17)：1702-1706,5.
Antitumor activity of F90,an epidermal growth factor receptor tyrosine kinase inhibitor,on glioblastoma cell line SHG-44
Antitumor activity of F90,an epidermal growth factor receptor tyrosine kinase inhibitor,on glioblastoma cell line SHG-44
LIU Fang-jun 1GUI Song-bai 2LI Chu-zhong 1SUN Ze-lin 1ZHANG Ya-zhuo1
作者信息
- 1. Beijing Neurosurgical Institute,Capital Medical University,Beijing 100050,China
- 2. Department of Neurosurgery,Beijing Tiantan Hospital,Capital Medical University,Beijing 100050,China
- 折叠
摘要
Abstract
Background Over-expression of epidermal growth factor receptor (EGFR) is thought to be related to cell proliferation,invasion,metastasis,resistance to chemoradiotherapy and poor prognosis of various human cancers.Forty percent to fifty percent of glioblastoma multiforme (GBM) possess deregulated EGFR,which may contribute to the aggressive and refractory course of GBM.Therefore,blockade of EGFR signal transduction may be a promising treatment strategy for GBM.Methods MIT assay,cell growth curve assay and tumor xenograft model were used to evaluate the antitumor activity of F90 against SHG-44 in vitro and in vivo.Western blot assay was applied to evaluate the expression of p-EGFR,p-ERK1,p-JNK,p-P38,Bcl2 and P53 proteins.Results F90 inhibited the cell proliferation in a dose-dependent manner in vitro.The growth of SHG-44 tumor xenografts was suppressed by F90 at a high dose level (100 mg.kg-1.d-1).Phosphorylation of EGFR and activated downstream signaling proteins,such as ERK1,JNK and P38,were found to be depressed after incubation with F90 for 48 hours in vitro.Down-regulated Bcl2 protein and up-regulated P53 protein were also observed.Conclueions The results demonstrate that F90 is effective in inhibiting the proliferation of SHG-44 cells in vitro and tumor growth in vivo,suggesting that F90 may be a new therapeutic option for treatment of GBM.关键词
glioblastoma/epidermal growth factor receptor/lressaKey words
glioblastoma/epidermal growth factor receptor/lressa分类
医药卫生引用本文复制引用
LIU Fang-jun,GUI Song-bai,LI Chu-zhong,SUN Ze-lin,ZHANG Ya-zhuo..Antitumor activity of F90,an epidermal growth factor receptor tyrosine kinase inhibitor,on glioblastoma cell line SHG-44[J].中华医学杂志（英文版）,2008,121(17):1702-1706,5.
