首页|期刊导航|中华医学杂志（英文版）|Growth inhibition and gene induction in human hepatocellular carcinoma cell exposed to sodium 4-phenylbutanoate
中华医学杂志（英文版）2008，Vol.121Issue(17)：1707-1711,5.
Growth inhibition and gene induction in human hepatocellular carcinoma cell exposed to sodium 4-phenylbutanoate
Growth inhibition and gene induction in human hepatocellular carcinoma cell exposed to sodium 4-phenylbutanoate
摘要
Abstract
Background Sodium 4-phenylbutanoate (NaPB) can induce cellular differentiation and cell cycle arrest.However,its potential anticancer properties in hepatocellular carcinoma and influence on normal liver cell are still unclear.We observed the effects of NaPB on growth inhibition,including differentiation and phase growth arrest in normal liver cell line L-02 and hepatocellular carcinoma cell line Bel-7402.Furthermore,we investigated its mechanism in Bel-7402.Methods Hepatocellular carcinoma cells Bel-7402 and normal liver cell line L-02 were treated with NaPB at different concentrations.Light microscopy was used to find morphological change in cells.Cell cycle was detected by flow cytometry.Expression of acetylating histone H4 and of histones deacetylase 4 (HDAC4) were determined by Western blot.The expression of P21WAF1/CIP1 and E-cadherin were observed through immunocytochemistry.Results NaPB treatment led to time dependent growth inhibition in hepatocellular carcinoma cells Bel-7402.NaPB treatment caused a significant decline in the fraction of S phase cells and a significant increase in G0/G1 cells.NaPB increased the expression of P21WAF1/CIP1 and E-cadherin in Bel-7402 and significantly decreased the level of HDAC4 in Bel-7402.NaPB significantly improved the level of acetylating histone H4.The normal liver cell line L-02 showed no distinct changes under treatment with NaPB.Conclusions NaPB inhibited the growth of hepatocellular carcinoma cells Bel-7402 and induced partial differentiation through enhancing the acetylating histones.In Bel-7402,the expressions of P21WAF1/CIP1 and E-cadherin may be related to level of acetylating histones and inhibition of cellular growth.NaPB showed no significant effect on normal liver cells.关键词
sodium 4-phenylbutyrate/histones/hepatocellular carcinoma/cell lineKey words
sodium 4-phenylbutyrate/histones/hepatocellular carcinoma/cell line分类
医药卫生引用本文复制引用
WANG Chun-ting,,MENG Mei,ZHANG Ji-cheng,JIN Chang-jun,JIANG Jin-jiao,REN Hong-sheng,JIANG Jun-mei,QIN Cheng-yong,YU Dong-qing..Growth inhibition and gene induction in human hepatocellular carcinoma cell exposed to sodium 4-phenylbutanoate [J].中华医学杂志（英文版）,2008,121(17):1707-1711,5.基金项目
This research was supported by grants from the Department of Science and Technology of Shandong Province (No.2005GG4402049) and Department of Health of Shandong Province. （No.2005GG4402049）
