中国临床康复2006，Vol.10Issue(34)：171-173,3.
社会环境压力对中国树鼩血液学和骨髓象指标的影响
Effects of the social environment stress on the indexes of hematology and myelogram in Chinese tree shrews
摘要
Abstract
BACKGROUND: The stress can induce sympathic nervous-adrenalmedulla system to release a lot of noradrenalin(NE) and adrenalin(E),pro mote bone marrow hemopoietic stem cells to differentiate towards lympho cyte and plasmocyte,decrease the number of granulocyte,thus organism can adapt to surrounding stress,so as to protect critical organs from damage. OBJECTIVE: To observe effect of confinement and vibration stress on in dexes of hematology and myelogram in Chinese tree shrews. DESIGN: Sex-marched and controlled experiment. MATERIALS: Thirty Chinese tree shrews (15 females, 15 males) weigh ing 120 g to 140 g were selected. Self-made experimental cylinders with many vent-holes, 16 cm length and 6cm inside diameter,were used, one side of which was sealed with plexglass,and the other side was blocked by mov able plexiglass for animals in and out.The air bath and homothermal vibra tor(Type SHZ-82,made in Changzhou,China). METHODS: The experiment was completed at the Experimental Center of Public Security, Gansu Political Science and Law Institute from March to October in 2003. ①Thirty Chinese tree shrews were randomly divided into five groups(n=6):The animals in the control group(C group) were fed nor mally; The experimental groups consisted of reserpine + vibration and vi bration groups. The animals in the reserpine + vibration (R+V)group were administered reserpine (0.05 mg/kg, im.) and vibrated at 140 beats/min; the vibration groups (V1,V2,V3) were vibrated at 70, 140, 210 beats/min, respectively. ② The animals in experimental groups were individually kept in the experimental tubes for 5 minutes, then put the tubes into the air bath and homothermal vibrator for 2 minutes. ③ The bleeding time, blood sedimentation, clotting time, and indexes of hemogram were determined with the methods of Duke, Westergren, glass capillary, and full-automatic biochemical instruments (type 721, Japan), respectively. ④ Dunnett ANO- VA was used to compare the differences between groups . MAIN OUTCOME MEASURES: Comparison of indexes of hematology and myelogram in Chinese tree shrews betweem the four groups.RESULTS: Thirty Chinese tree shrews were involved in the final analysis.① Indexes of hematology: compared with those of C group, the bleeding time of the V2 group significantly prolonged by 104.8% (P＜0.01 ),but the clotting time and blood sedimentation decreased by 37.3% and 7.5% (P＜0.01 ), respectively. Also, the counts of red blood cells, reticulocyte, blood platelet and white blood cells,as well as content of hemoglobin increased by i7.5% ,50.9%, 11.8%, 10.3% and 13.7% ,respectively.There was no significant difference between R+V group and C group(P ＞0.05),and also in various vibration groups. ②Indexes of myelogram:compared with those of C group,counts of granulocyte were decreased by 31.5%(P＜0.01),counts of red cells,lymphocyte,mononuelear,plasmocyte,megakaryocyte,mitotic cells increased by 25.7% ,77.6%,213.3% ,65.8% ,100%,161.3%,respectively (P＜0.01); those in R+V group were similar to C group (P＞0.05); There were no significant differences among various vibration groups(P＞0.05).CONCLUSION: Confinement and vibration stress can increase significantly the blood cells and reduce counts of granulocyte in bone marrow,and the above changes can be interfered by reserpine.关键词
应激/骨髓检查/血细胞计数分类
医药卫生引用本文复制引用
俞发荣,连秀珍..社会环境压力对中国树鼩血液学和骨髓象指标的影响[J].中国临床康复,2006,10(34):171-173,3.基金项目
甘肃政法学院"135人才工程"基金项目(GZF04-75) （GZF04-75）
"135 Talents Project"Foundation of Gansu Political Science and Law Intitute,No. GZF04-75 （）
