中国当代儿科杂志2004，Vol.6Issue(3)：171-175,5.
川芎嗪对哮喘大鼠气道壁胶原沉积和TGF-β1表达的影响
Effect of ligustrazine on the levels of collagen and transforming growth factor-β1 in rats with asthma
王文建 1杨莉 1王西华 1李海浪1
作者信息
- 1. 无锡市儿童医院呼吸科,江苏,无锡,214002
- 折叠
摘要
Abstract
Objective The formation of subepithelial fibrosis and increased deposition of types Ⅰ and Ⅲ collagen are important features of airway remodelling in asthma, while transforming growth factor-β (TGF-β1) is the major regulative factor of airway remodelling. The study aims at exploring the effect of ligustrazine on the collagen deposition and the expression of TGF-β1 in the airway wall of asthmatic rats. Methods Forty SD rats were randomly assigned into 5 groups (n=8 each): a normal group (A), an asthma model group (B), a dexamethasone group (C), a low-dose ligustrazine group (D) and a high-dose ligustrazine group (E). The asthma model was established by repeated inhalation of oval bulium. Groups C, D and E were treated by injection of 0.5 mg dexamethason and 40 mg or 80 mg ligustraizine respectively before stimulation, while Group A was treated with normal saline instead. The levels of collagen and TGF-β1expression in airway wall were semi-quantitatively measured by the irmmunohisto-chemistry technique. Results The levels of collagen type Ⅲ and TGF-β1 expression were significantly higher in Group B than those in Group A ( P ＜0.01). In the Group C, Group D and Group E, the levels of collagen type Ⅲ and TGF-β1 expression in airway wall were lower than those in Group B ( P ＜0.01), and there was no difference among Groups E, C and A ( P ＞0.05). The contents of collagen type Ⅰ had no difference among all five groups. A close correlation between TGF-β1 expression and collagen type Ⅲ content in various groups was found ( rs = 0. 7063, P ＜ 0.01). Conclusions Ligustrazine may reduce the deposition of collagen type Ⅲ and inhibit the expression of TGF-β1.关键词
川芎嗪/哮喘/胶原/转化生长因子β1/大鼠Key words
Asthma/Ligustrazine/Collagen/Tranforming growth factor-β1/Rat分类
医药卫生引用本文复制引用
王文建,杨莉,王西华,李海浪..川芎嗪对哮喘大鼠气道壁胶原沉积和TGF-β1表达的影响[J].中国当代儿科杂志,2004,6(3):171-175,5.
