动物学研究2009，Vol.30Issue(2)：171-177,7.DOI:10.3724/SP.J.1141.2009.02171
抗菌肽OH-CATH对大肠杆菌Escherichia coli的作用
Effects of the Antimicrobial Peptide OH-CATH on Escherichia coil
摘要
Abstract
OH-CATH is a novel cathelicidin identified from king cobra. It showed strong antibacterial activity against various bacteria in the presence of 1% NaCi and no haemolytic activity toward human red blood cells even at a high concentration. OH-CATH might serve as model molecules for the development of antimicrobiai drugs. Understanding the action mechanism of OH-CATH and the reason for its selectivity against microbes is very important for this purpose. The bactericidal effect of the king cobra antimicrobial peptide OH-CATH on Gram-negative Escherichia coli (ATCC 25922) is observed by scanning electron microscopy (SEM) and transmitted electron microscopy (TEM). The SEM and TEM results suggested that the bactericidal mechanism of OH-CATH against Escherichia coil happened in three steps. Firstly, OH-CATH attached to the negatively charged bacterial wall by positively charged amino acid residues. In the second step, the accumulated OH-CATH aggregated and damaged the bacteria membrane in a pore-forming manner. In the last step, with the damage of cell permeability, the contents of the cells were released and eventually cells died.关键词
抗菌肽/OH-CATH/大肠杆菌/电镜Key words
Antimicrobial peptide/OH-CATH/Escherichia coli/Electron microscopy分类
生物科学引用本文复制引用
陈欣欣,余果宇,张彦,张云,申吉泓,李文辉..抗菌肽OH-CATH对大肠杆菌Escherichia coli的作用[J].动物学研究,2009,30(2):171-177,7.基金项目
国家自然科学基金(30670412) （30670412）
中国科学院重要方向项目(KSCX2-YW-R-088):云南省自然科学基金资助项目(2005PY01-23:2006PT08-1:2006C0047Q) （KSCX2-YW-R-088）
