医学新知杂志2001，Vol.11Issue(1)：17-19,3.
慢性乙型肝炎患者乙型肝炎病毒C基因启动子变异的初步研究
Pilot Study on Hepatitis B Virus Core Promoter Mutations in Patients
张斌 1万谟彬 1潘欣 2李成忠 1陈志辉 1张迁 1戚中田2
作者信息
- 1. 第二军医大学附属长海医院感染科 上海 200433
- 2. 第二军医大学微生物教研室 上海 200433
- 折叠
摘要
Abstract
To establish PCR for detection hepatitis B virus (HBV) DNA andDetect hepatitis B virus core promoter (CP) mutations in patients with hepalitis B,polymerase chain reaction amplified HBV DNA fragments were directly sequenced and common-source analyzed with the help of computer. The results showed that the positive rate of serum samples from 20 HB patients was 95%(19/20).The HBV CP fragments of two patients and pGEM.7 Z-HBV plasmid were sequenced. Compared with report gene sequence,the identical rate of 2 patients were only 72.0%,66.5% respectively,but pGEM. 7 Z-HBV plasmid was 90.0%.This study indicated that PCR may be used in the detection of HBV with mutations in CP region and CP region mutation exists in patient with chronic hepatitis B.关键词
乙型肝炎病毒/C基因启动子/变异Key words
Hepatitis B virus/Core promoter/Mutation分类
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张斌,万谟彬,潘欣,李成忠,陈志辉,张迁,戚中田..慢性乙型肝炎患者乙型肝炎病毒C基因启动子变异的初步研究[J].医学新知杂志,2001,11(1):17-19,3.
