高技术通讯（英文版）2004，Vol.10Issue(2)：17-20,4.
Cloning and Characterization of Gene Promoters from Bacillus pumilus
Cloning and Characterization of Gene Promoters from Bacillus pumilus
摘要
Abstract
DNA fragments obtained from Sau3AI partially digested total DNA of Bacillus pumilus UN31-C-42 are first inserted into BamHI site of pSUPV4, a promoter-probe vector. The recombinant DNA molecules are transformed into Escherichia coli cells and eight-three Kanr clones (named pSUBp1- pSUBp83) are obtained. The inserted fragments in pSUBp53, pSUBp57, pSUBp21, which showed high level of kanamycin - resistance, are sequenced and analyzed, respectively. These fragments contain some conserved sequences of prokaryotic gene promoters, such as TATAAT and TTGACA box. The promoter fragment Bp53 could efficiently promote the alkaline protease gene of B.pumilus expression not only in E.coli but also in B.subtilis cells.关键词
Bacillus pumilus/Escherichia coli/Bacillus subtilis/promoter cloning/gene expression/promoter-probe vectorKey words
Bacillus pumilus/Escherichia coli/Bacillus subtilis/promoter cloning/gene expression/promoter-probe vector分类
信息技术与安全科学引用本文复制引用
Pan Jiao(潘皎),Zhang Yizheng..Cloning and Characterization of Gene Promoters from Bacillus pumilus[J].高技术通讯（英文版）,2004,10(2):17-20,4.基金项目
Supported by the High Technology Research and Development Programme of China. （）
