中国药房2001，Vol.12Issue(3)：172-173,2.
高效液相色谱法测定羟基喜树碱及喜树碱含量
The Determination of Hydroxycamptothecinum and Camptothecinum by HPLC
马锦星 1孙仲葆 1彭才勤 2马烈2
作者信息
- 1. 湖北省黄石市药品 检验所,
- 2. 武警湖北总队医院,
- 折叠
摘要
Abstract
OBJECTIVE： To establish a HPLC assay for the determination of hydroxycamptothecinum and camptothecinum.METHODS： The concentrations of hydroxycamptothecinum and camptothecinum were assayed on a C18 column with a mobile phase consisting of methanol－ water(45∶ 55) at a flow rate of 1.0 ml/min,and detected at 266nm.RESULTS： The mean recovery of hydroxycamptothecinum was 100.2％ ,the 2,4,8 hours precision RSD were less than 1.8％ ;The mean recovery of camptothecinum was 99.7％ ,the 2,4,8 hours precision RSD were less than 2.0％ .CONCLUSION： The HPLC method for determination of hydroxycamptothecinum and camptothecinum was rapid,sensitive and accurate.
关键词
HPLC/羟基喜树碱/喜树碱分类
医药卫生引用本文复制引用
马锦星,孙仲葆,彭才勤,马烈..高效液相色谱法测定羟基喜树碱及喜树碱含量[J].中国药房,2001,12(3):172-173,2.
