中国现代医学杂志2005，Vol.15Issue(2)：172-176,5.
三七总皂甙预处理大鼠供肝对细胞凋亡及TNF-α、Caspase-3表达的影响
Impact of panax notoginseng saponins preconditioning on apoptosis and the expression of TNF-α, Caspase-3 in rat liver transplantation
张毅 1叶启发 2明英姿 2许贤林 1肖建生 1李远明2
作者信息
- 1. 华中科技大学附属同济医院,器官移植研究所,湖北,武汉,430030
- 2. 中南大学,湘雅三医院移植医学研究院,湖南,长沙,410013
- 折叠
摘要
Abstract
[Objective] To investigate the protective effects and the impact on the expression of TNF-α and Caspase-3 mRNA of panax notoginseng saponins (PNS) preconditioning in rat liver transplantation. [Methods] Male Sprague-Dawley rats were used as donors and recipients of orthotopic liver transplantation (OLT) and were divided into PNS preconditioning group (group P) and NS control group (group N) randomly according to whether PNS was injected by venous (50 mg/kg) 1 h before liver grafts harvesting. The animals were separately killed at 2 h, 6 h and 24 h after reperfusion. Plasma samples were collected for ALT and AST test. Liver tissues were collected to detect histological changes, apoptosis and the expression of TNF-α and Caspase-3 mRNA. [Results] The serum levels of ALT and AST and the apoptosis index (AI) of liver tissue in group P were lower than group N significantly (P ＜0.05)at 2 h, 6 h and 24 h after reperfusion. The expression of TNF-α and Caspase-3 mRNA was decreased significantly in group P 2 h, 6 h after reperfusion (P ＜0.05). [Conclusion] Panax notoginseng saponins preconditioning could attenuate liver grafts ischemia/reperfusion injury and apoptosis of hepatocytes. Affecting expression of TNF-α and Caspase-3 genes may one of mechanisms of PNS antiapoptotic effects.关键词
三七总皂甙/鼠/肝移植/TNF-α/Caspase-3Key words
panax notoginseng saponins/rat/liver transplantation/TNF-α/Caspase-3分类
医药卫生引用本文复制引用
张毅,叶启发,明英姿,许贤林,肖建生,李远明..三七总皂甙预处理大鼠供肝对细胞凋亡及TNF-α、Caspase-3表达的影响[J].中国现代医学杂志,2005,15(2):172-176,5.
