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QTL Mapping of Chlorophyll Contents in Rice

SHEN Bo ZHUANG Jie-yun ZHANG Ke-qin DAI Wei-min LU Ye FU Li-qing DING Jia-ming ZHENG Kang-le

中国农业科学(英文版)2007,Vol.6Issue(1):17-24,8.
中国农业科学(英文版)2007,Vol.6Issue(1):17-24,8.

QTL Mapping of Chlorophyll Contents in Rice

QTL Mapping of Chlorophyll Contents in Rice

SHEN Bo 1ZHUANG Jie-yun 2ZHANG Ke-qin 1DAI Wei-min 1LU Ye 1FU Li-qing 2DING Jia-ming 2ZHENG Kang-le2

作者信息

  • 1. National Center for Rice Improvement and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R. China
  • 2. College of Biological Science, Hangzhou Normal College, Hangzhou 310012, P.R. China
  • 折叠

摘要

Abstract

The objectives of this study were to investigate the genetic factors controlling the chlorophyll content of rice leaf using QTL analysis. A linkage map consisting of 207 DNA markers was constructed by using 247 recombinant inbred lines (RILs) derived from an indica-indica rice cross of Zhenshan97B×Milyang 46. In 2002 and 2003, the contents of chlorophyll a and b of the parents and the 247 RILs were measured on the top first leaf, top second leaf, and top third leaf, respectively. The software QTLMapper 1.6 was used to detect quantitative trait loci (QTLs), additive by environment (AE) interactions, and epistatic by environment (AAE) interactions. A total of eight QTLs in four intervals were detected to have significant additive effects on chlorophyll a and b contents at different leaf positions, with 1.96-9.77% of phenotypic variation explained by a single QTL, and two QTLs with significant AE interactions were detected. Epistasis analysis detected nine significant additive-by-additive interactions on chlorophyll a and b contents, and one pair of QTLs with significant AAE interactions was detected. On comparison with QTLs for yield traits detected in the same population, it was found in many cases that the QTLs for chlorophyll a and b contents and those for yield traits were located in the same chromosome intervals.

关键词

rice/quantitative trait loci/chlorophyll content/yield traits/QTL×environment interaction

Key words

rice/quantitative trait loci/chlorophyll content/yield traits/QTL×environment interaction

分类

农业科学

引用本文复制引用

SHEN Bo,ZHUANG Jie-yun,ZHANG Ke-qin,DAI Wei-min,LU Ye,FU Li-qing,DING Jia-ming ,ZHENG Kang-le..QTL Mapping of Chlorophyll Contents in Rice[J].中国农业科学(英文版),2007,6(1):17-24,8.

基金项目

This study was supported by the National 863 Program (2003AA207030), the Key Program of Zhejiang Province, China (010007015), and the China Postdoctoral Science Foundation (2003034232). (2003AA207030)

中国农业科学(英文版)

2095-3119

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