中国临床康复2006，Vol.10Issue(9)：173-175,3.
慢性压迫性脊髓损伤后神经前体细胞的增殖
Proliferation of neural progenitor cell after chronic compressive injury of spinal cord
张绍文 1王栓科 1王翠芳 1夏亚一 1张海鸿 1汪玉良 1孙正义1
作者信息
- 1. 兰州大学第二医院骨科研究所,甘肃省兰州市,730030
- 折叠
摘要
Abstract
BACKGROUND: There is still no affirmative conclusion on the proliferative characteristics and the sources of neural progenitor cells after chronic compressive injury of spinal cord in adult mammals and the effects of astrocytes in this process.OBJECTIVE: To investigate the proliferative characteristics and the sources of neural progenitor cell and the effects of astrocytes by means of analyzing the changes of expression of nestin and glial fibrillary acidic protein after chronic compressive injury of spinal cord and after decompression in adult rats.DESIGN: Completely randomized control trial.SETTING: Orthopaedics Research Institute, the Second Hospital of Lanzhou University.MATERIALS: The experiment was completed in Orthopaedics Research Institute of the Second Hospital of Lanzhou University from March to October 2003. A total of 50 adult healthy Wistar rats were selected and randomly divided into normal control group, moderate chronic compressive spinal cord injury group (compressive mass occupied 40% of the diameter of spinal canal), severe compression group (compressive mass occupied 60% of the diameter of spinal canal). Three-day and 10-day decompression groups (depression after 24-hour severe compressive injury) with 10 in each group.MAIN OUTCOME MEASURES: ① Grey value of positive expression of nestin in grey and white matter in spinal cord segment near compression (5 mm to the edge of compression) in rats of each group. ② Expression of glial fibrillary acidic protein in spinal cord of rats in each group.RESULTS: All the 50 rats entered experimental analysis. ①There were significant expressions of nestin in moderate compression group (white matter 235.33±6.48, grey matter 196.28±6.55), severe compression group (white matter 190.45±4.91, grey matter 173.15±5.98), 3-day decompression after severe compressive injury group (white matter 198.39±3.24, grey matter 180.38±4.51) and 10-day decompression group (white matter 202.55±3.54) (P ＜ 0.05), especially in severe compression group (P ＜ 0.01).Compared with the normal control group, the difference between the ex pression of nestin in grey matter and that in ependymal cells on the central canal of spinal cord in 10-day decompression group has no significance (P ＞ 0.05). ②Compared with normal control group, the expression of glial fibrillary acidic protein in spinal cord increased in each injury group,and the amount of positive cells of glial fibrillary acidic protein went up and cell soma was hypertrophic, and the processes became thicker and longer.CONCLUSION: There is neural progenitor cell proliferation in the early stage of chronic compressive injury of spinal cord and after decompression in adult rats. Astrocyte participates in proliferation and migration of neural progenitor cells and has important trophic and repair effects on spinal cord.关键词
脊髓损伤/神经元/神经胶质原纤维酸性蛋白质分类
医药卫生引用本文复制引用
张绍文,王栓科,王翠芳,夏亚一,张海鸿,汪玉良,孙正义..慢性压迫性脊髓损伤后神经前体细胞的增殖[J].中国临床康复,2006,10(9):173-175,3.
