中药材2001，Vol.24Issue(3)：174-175,2.
SFE-RP-HPLC法测定金毛狗脊中磷脂含量
Determination of Phoosphlipid in barometz By Supercritical Muid Ectraction and RP-HPLC
许重远 1陈志良 1陈振德 1葛发欢 2张焜3
作者信息
- 1. 第一军医大学南方医院广州510515
- 2. 广州市医药工业研究所广州510240
- 3. 广东工业大学轻化学院广州510000
- 折叠
摘要
Abstract
A supereritical fluid extraetion (SFE) method was used to extractphospholipids in Cibotium barometz. After a homogeneous design experiment, SFE conditions were obtained which were pressure at 31.5 MPa, temperature at 46.2℃ and a 0.37 L/kg modifier in supercritical fluid. The sample was extracted for 2 hours. A RPLC method was developed for the determination of phosphatidylcholine. RPHPLC conditions included a RP-HPLC column packed with Waters Symmetry C18(5 μm), a mobile phase composed of methanol-1% phosh poric acid(90:10) and the detection wavelength at 206 nm. The content of phosphatidylcholine was 0.198%(RSD 4.2%), and the method can be a measure of quality control for Cibotium barormetz and its preparations.关键词
超临界流体萃取/反相高效液相色谱法/金毛狗脊磷脂分类
医药卫生引用本文复制引用
许重远,陈志良,陈振德,葛发欢,张焜..SFE-RP-HPLC法测定金毛狗脊中磷脂含量[J].中药材,2001,24(3):174-175,2.
