中德临床肿瘤学杂志（英文版）2009，Vol.8Issue(3)：175-177,3.DOI:10.1007/s10330-008-0165-1
葡萄球菌肠毒素A联合PML-RAR α多肽刺激特异性T细胞杀伤NB4细胞株的作用
Stimulation of staphylococcal enterotoxin A combined with PML-RARa peptide on the specifical T-cells against NB4 cell line
摘要
Abstract
Objective: To investigate the effects of staphylococcal enterotoxin A (SEA) on the cytotoxicity of T cells stimulated by PML-RARa peptide in vitro. Methods: Peripheral blood mononuclear cells (MNC) from healthy donor were obtained by density gradient cantrifugation on Ficoll-Hypaque, MNC were cultured with PML-RARa peptide and SEA for 20 days. After induction, the cytotoxicity of T cells induced against NB4 and K562 cell lines were examined by Cell Counting Kit-8 (CCK-8).The CD4 and CD8 surface markers on the harvested CD3+ T cells were detected by flow cytometry (FCM). Results: The cytotoxicity of T cells induced by PML-RARa peptide with SEA was higher than that of T cells induced only by PML-RARa peptide against NB4 cells. The FCM assay showed that the ratio of CD4+/CD8+ T cells were gradually decreased in both groups of PML-RARa peptide whether with SEA or not at the intervals of day 5,10 and 20 day after induction, but the most significantly decreased by PML-RARa peptide with SEA. Conclusion: The specific cytotoxicity of T cells induced by PML-RARa peptide against NB4 cells could be enhanced with superantigen SEA.关键词
superantigen/PML-RARα peptide/NB4 cells/T cellsKey words
superantigen/PML-RARα peptide/NB4 cells/T cells分类
医药卫生引用本文复制引用
Chen Lin,Xue Bai,Lijian Yang,Shaohua Chen,B. N. Selvakumar,Yangqiu Li..葡萄球菌肠毒素A联合PML-RAR α多肽刺激特异性T细胞杀伤NB4细胞株的作用[J].中德临床肿瘤学杂志（英文版）,2009,8(3):175-177,3.基金项目
Supported by grants from the Science and Technology Commission of Guangdong Province (No. 06025169, No. 2005B50301016), and the Key Laboratory of Pathophysiology of Jinan University. （No. 06025169, No. 2005B50301016）
