首页|期刊导航|农业科学学报（英文）|Production and Characterization of Polyclonal Antibody for the N-Methylcarbamate Insecticide Metolcarb
农业科学学报（英文）2007，Vol.6Issue(2)：175-179,5.
Production and Characterization of Polyclonal Antibody for the N-Methylcarbamate Insecticide Metolcarb
Production and Characterization of Polyclonal Antibody for the N-Methylcarbamate Insecticide Metolcarb
摘要
Abstract
The hapten, 3-{[1-(3-(methyl)phenyloxy)-carbonyl]amino}propanoic acid (HOM), mimicking the analyte metolcarb, was synthesized and verified by mass spectrometry (MS) and 1H-nuclear magnetic resonance spectrometry (1H-NMR). Then,HOM was conjugated with the carrier proteins bovine serum albumin (BSA) and ovalbumin (OVA) with stoichiometric amounts of N-hydroxysuccinimide/dicyclohexylcarbodimide (NHS/DCC) using the activated ester method. Polyclonal antibodies were raised against the conjugate of HOM-BSA in rabbits. Antiserum titres were determined by noncompetitive indirect ELISA procedures and the titer of pAb01 reached 1.28 × 106. The cross-reactivities of the structurally related Nmethylcarbamate insecticides were 0.0% except for dimethacarb. These results indicate that the antibody pAb01 with strong affinity and high specificity can be used to develop a sensitive and rapid detection protocol for metolcarb residue.关键词
metolcarb/antigen/polyclonal antibody/antiserum characterization/ELISAKey words
metolcarb/antigen/polyclonal antibody/antiserum characterization/ELISA分类
农业科技引用本文复制引用
ZHANG Qi,LI Tie-jun,ZHU Xiao-xia,XU Li-na,LIU Feng-quan,HU Bai-shi,JIANG Ying-hua,CAO Bin..Production and Characterization of Polyclonal Antibody for the N-Methylcarbamate Insecticide Metolcarb[J].农业科学学报（英文）,2007,6(2):175-179,5.基金项目
This study was supported by the National High Technology Research and Development Program (863 Program), China (2006aa10z447) and Important Technology in Agricultural Frame Adjusting of Ministry of Agriculture, China (2003-07-02A). （863 Program）
