中国临床康复2004，Vol.8Issue(1)：176-177,2.
骨形成蛋白4诱导成年大鼠骨髓间充质干细胞分化为神经元的能力
Bone morphogenetic protein 4 induces the differentiation of adult rat bone marrow-derived mesenchymal stem cells into neurons
摘要
Abstract
AIM:To evaluate the ability of bone morphogenetic protein 4 (BMP4) in inducing the in vitro differentiation of adult rat bone marrow-derived mesenchymal stem cells (MSCs) into neurons. METHODS:Using immunohistochemichal method,the expressions of neurofilament protein(NF) and glial fibriliary acidic protein (GFAP) were detected in BMP4-induced MSCs of the fifth passage. RESULTS:BMP4-induced MSCs were transformed into neuron-like cells,which were positive for NF expression as shown by immunocytochemical determination.The percentage of NF-positive neuron-like cells at 5 h,12 h,1 d, 3 d, 5 d and 7 d after induction was (40± 7)% ,(71± 6)% ,(58± 3)% ,(53± 6)% ,(37± 5)% and (13± 2)% respectively.The peak of NF expression in the neuron-like cells occurred at 12 h after induction,which was higher than that 5 h after induction(t=1.380- 2.621,P< 0.05). CONCLUSION:BMP4 can induce rat bone marrow-derived MSCs into neurons in vitro.关键词
骨髓/干细胞/神经元/细胞分化分类
医药卫生引用本文复制引用
陈东风,杜少辉,李伊为,黎晖..骨形成蛋白4诱导成年大鼠骨髓间充质干细胞分化为神经元的能力[J].中国临床康复,2004,8(1):176-177,2.基金项目
国家自然科学基金项目(30271677,30371837) （30271677,30371837）
