解放军医学杂志2001，Vol.26Issue(3)：176-178,3.
环孢菌素A致大鼠肾小管间质细胞凋亡及Losartan、Enalapril的保护作用
APOPTOSIS INDUCED BY CYCLOSPORINE A IN RENAL TUBULAR AND INTERSTITIAL CELLS AND THE PROTECTIVE EFFECTS OF ENALAPRIL AND LOSARTAN ON IT
张国华 1张训 1侯凡凡 1王力 1易朝辉1
作者信息
摘要
Abstract
The rats were fed on low salt diet for seven days,and then were randomly divided into five groups: control (n=7), CsA treated (n=7), CsA+Verapamil (n=7), CsA+Enalapril (n=7) and CsA+Losartan (n=7).CsA was given by subcutaneous injection (15mg*kg-1*d-1) for four weeks. The apoptosis of tubular and interstitial cells was detected by TUNEL assay. The proliferating cell nuclear antigen and Fas antigen expression were examined by immunohistochemical staining. Apoptosis of tubular and interstitial cells increased in CsA treated rats. CsA+Losartan and CsA+Enalapril treated rats had a statistically significant decrease in apoptosis when compared to CsA treated rats (7.3±1.1 and 6.6±0.8 vs 13.9±1.2 respectively, P<0.01 in all)，and the tubular Fas antigen expression in CsA + Losartan or CsA+ Enalapril group was significantly lower than in CsA treated group(19.8±2.4 and 18.2±1.8 vs 37.8±5.7 respectively, P<0.01 in all).The results showed that CsA nephropathy was associated with marked increase in apoptosis of tubular and interstitial cells. Fas may be an important mediator of tubular apoptosis induced by CsA. Enalapril and Losartan are effective on decreasing the number of tubular and interstitial apoptosis cells and expression of tubular Fas antigen.关键词
环孢菌素/细胞凋亡/血管紧张素/肾小管间质纤维化/Fas 抗原分类
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张国华,张训,侯凡凡,王力,易朝辉..环孢菌素A致大鼠肾小管间质细胞凋亡及Losartan、Enalapril的保护作用[J].解放军医学杂志,2001,26(3):176-178,3.
