中国现代医学杂志2006，Vol.16Issue(12)：1766-1771,6.
融合自杀基因CDglyTK治疗喉癌的体外实验研究
Study of treating laryngeal cancer with suicide fusion gene CDglyTK in vitro
摘要
Abstract
[Objective] To study the in vitro treatment of laryngeal cancer using suicide gene CDglyTK. [Methods] Plasmid pcDNA3.1(-)CMV.CDglyTK, constructed by PCR, enzyme digestion, ligation and transduction etc was verified by digesting of Xho Ⅰ/Hind Ⅲ and automatic sequence analysis, then it was introduced into Hep-2 cells by electroporation to yield cells expressing CDglyTK stably after selecting with G418 (400 μg/mL) for 14 days. The expression of CDglyTK mRNA in transfected Hep-2 cells was tested by RT-PCR.In vitro chemosensitivity of CDglyTK -expressing Hep-2 cells to 5-FC, GCV or 5-FC +GCV was detected by MTT assay. [Results] The recombinant plasmid contained full-length coding region sequence of CD and TK gene. A anticipated 707 bp fragment was amplified from total RNA of CDglyTK-expressing Hep-2 cells by RT-PCR and a fusion protein of 59 kDa was detected in cell extract from transfected Hep-2 cells. In vitro study growth of CDglyTK-positive Hep-2 cells were inhibited by 5-FC, GCV or 5-FC +GCV respectively,and the antitumour effect of 5-FC +GCV is superior to 5-FC or GCV.[ Conclusion] CDglyTK may be a candidate for treating human laryngeal cancer.关键词
CDglyTK/基因治疗/喉癌Key words
CDglyTK/gene therapy/laryngeal cancer分类
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唐瑶云,徐婧,刘建平,郑颂扬,赵素萍,张俊毅,肖健云..融合自杀基因CDglyTK治疗喉癌的体外实验研究[J].中国现代医学杂志,2006,16(12):1766-1771,6.基金项目
Supported by National Natural Science Foundation of China (NO:30300414) （NO:30300414）
