湖南农业大学学报（自然科学版）2001，Vol.27Issue(3)：179-181,3.
转基因抗虫油菜的ELISA分析
ELISA Analysis of Transgenic Insect-Resistant Rapeseed
摘要
Abstract
To select and breed a new variety of high yield and high resistance to insects,the expression of Bt insecticidal protein gene in the transgenic rapeseed plants was studied with ELISA.The results show that Bt insecticidal protein gene variedly expresses in the transgenic rapeseed plants with its growth stage.It stably and efficiently expresses during the 3rd～9th leaf stage;its expressing quantity is up to 6.68～10.52 ng/mg fresh leaf;its proportion to plant soluble protein is 0.067%～0.105%. But its expressing quantity apparently decreases after the 11th leaf stage to 0.28～2.00 ng/mg fresh leaf,and its proportion to plant soluble protein is 0.003%～0.020%.关键词
转基因油菜/Bt杀虫蛋白基因/酶联免疫吸附法分类
农业科技引用本文复制引用
林良斌,周小云,官春云,王学军..转基因抗虫油菜的ELISA分析[J].湖南农业大学学报（自然科学版）,2001,27(3):179-181,3.基金项目
湖南省科技厅资助项目(97JKY1005) （97JKY1005）
