华西医科大学学报2001，Vol.32Issue(2)：179-182,4.
中国人Ⅱb型高脂蛋白血症apoE基因多态性的研究
Study on apoE Gene Polymorphism in Chinese Type Ⅱb Hyperlipidemia
摘要
Abstract
Objective To study apolipoprotein(apo) E polymorphism and its relationship with plasma lipids and apolipoproteins levels in Chinese patients with type Ⅱb hyperlip oproteinemia. Methods apoE genotypes were assayed by polymerase chain reaction(PCR)-restriction fragment length polymorphism(RFLP) method,serum lipids were determined by enzyme method, and apolipoproteins were measured by radial immunodiffusion assay in 74 type Ⅱb hyperlipidemia patients whose fasting serum lipids levels were TG≥2.26mmol/L,TC ≥6.21 mmol/L and in 230 healthy subjects whose fasting serum lipids levels were TG<1.82mmol/L, TC<6.21 mmol/L from a population of Chinese Han nationality in Chengdu area. Results Compared with the control group， the serum TG，TC，LDLC， nHDLC， apoAⅡ， apoB100， apoCⅡ, apoCⅢ， apoE levels and TG/HDLC ratio in patients with type Ⅱb hyperlipidemia were significantly increased(P<0.001), and the serum HDLC levels and apoE/apoCⅢ ratio were significantly decreased(P<0.05). ApoE3/3 genotype and allele ε3 frequency in type Ⅱb hyperlipidemia group and control group were both the highest. In type Ⅱb hyperlipidemia group， allele ε2 frequency tended to increase and allele ε4 frequency tended to decrease as compared with those in the control group (P>0.05). In type Ⅱb hyperlipidemia group the genotype of apoE2 was associated with higher serum TG， apoCⅡ， apoE levels and apoE/apoCⅢ ratio when compared with the genotype of apoE3; the genotype of apoE4 was associated with higher serum TC， nHDLC and apoE levels when compared with the genotypes E3 and E2(P<0.001). Conclusion The alleles ε2 and ε4 are associated with serum TG，TC，nHDLC，apoCⅡ and apoE levels to some extent in type Ⅱb hyperlipide in Chinese population.关键词
Ⅱb型高脂血症 载脂蛋白E 等位基因 基因型 PCR-RFLP分类
医药卫生引用本文复制引用
张雪梅,白怀,刘秉文,范萍,张荣爵,徐燕华,姚佳,朱红..中国人Ⅱb型高脂蛋白血症apoE基因多态性的研究[J].华西医科大学学报,2001,32(2):179-182,4.基金项目
国家自然科学基金资助(批准号 39770322) （批准号 39770322）
