中国临床康复2006，Vol.10Issue(47)：180-182,188,4.
江浙蝮蛇毒纤溶酶的生化和药理性质特征
Biochemical and pharmacological characteristics of a fibrinolytic enzyme from Agkistrodon halys Pallas venom in Jiangzhe region
摘要
Abstract
BACKGROUND: A fibrinolytic enzyme (FLE) was purified from the venom of Agkistrodon blomhoffi brevicaudus by ion-exchange chromatography;meanwhile, it can dissolve thrombotic anti-thrombus drugs during onset of myocardial infarction and apoplexy.OBJECTIVE: To investigate the purification of FLE from the venom of Agkistrodon blomhoffi brevicaudus and research some biochemical and pharmacological characterizations.DESIGN: Controlled observational study.SETTING: Snake Venom Research Institute, Guangxi Medical University.MATERIALS: The experiment was carried out in Snake Venom Research Institute of Guangxi Medical University from July 2003 to June 2005.Kunming mice, weighing 18-25 g, of mean body mass of (20±2) g, aged 3-4 months were provided by Experimental Animal Center of Guangxi Medical University. Agkistrodon blomhoffi brevicaudus was supplied by Snake Venom Research Institute of Guangxi Medical University.METHODS: FLE was purified with DEAE Sepharose CL-6B ion exchang columns and HiPrep Sephacryl S 100; purity and relative molecular mass were measured with high-effective chromatograph of liquid and polyacrylamidedel electrophoresis (PAGE); isoelectric point was measured with isoelectric focusing electrophoresis; and then, components and partially pharmacological characteristics were also measured.MAIN OUTCOME MEASURES: Relative molecular mass, conformation of isoelectric point and amino acid, effect of inhibitor on FLE, hemorrhagic activity, and anticoagulant effect of FLE.RESULTS: All 30 rats and 12 rabbits were involved in the final analysis.Relative molecular mass was 59 100 and isoelectric point was 4.98. SDSPAGE acted as a zone in vitro. High-effective chromatograph of liquid showed a simple peak of FLE. This group belonged to metallo proteinases.Analysis of components of amino acid suggested that it contained a lot of acidity amino acids, had mild hemorrhagic toxicity and strongly thrombolytic effect.CONCLUSION: A simple acidity metallo proteinases, which is characterized by mild hemorrhagic toxicity and strong anti-coagulation, can be purified from the venom of Agkistrodon blomhoffi brevicaudus.关键词
纤溶酶/蛇毒液类/分离和提纯分类
医药卫生引用本文复制引用
张学荣,张箴波,舒雨雁,蒙怡,刘海涛..江浙蝮蛇毒纤溶酶的生化和药理性质特征[J].中国临床康复,2006,10(47):180-182,188,4.基金项目
广西壮族自治区科学技术厅资助项目(桂科基0342002-6) （桂科基0342002-6）
广西壮族自治区教育厅资助项目(桂教科研[2002]121)Supported by: the grants from Guangxi Scientific and Technological Bureau, No. 0342002-6* （桂教科研[2002]121）
the grants from Guangxi Educational Bureau, No. [2002] 121 （）
