中国动脉硬化杂志2009，Vol.17Issue(3)：181-184,4.
白细胞介素1β和肿瘤坏死因子α对大鼠主动脉平滑肌细胞内皮脂肪酶表达的影响
The Influence of Interleukin-1β and Tumor Necrosis Factor-α on Endothelial Lipase in Rat Aortic Smooth Muscle Cells
摘要
Abstract
Aim To investigate the effects of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) on endothelial lipase (EL) in rat aortic smooth muscle cell (ASMC). Methods The rat ASMC were obtained from thoracic aortas and cultured by tissue explant method. Passage 3～5 cells were used in experiment, and were randomly divided into 3 groups: Group Ⅰ: treated without intervention as the control;Group Ⅱ: the rat ASMC were treated in serumfree culture medium containing IL-1β (1 to 30 μg/L) for 24 hours ;Group Ⅲ: TNF-α (5 to 50 μg/L) for 24 hours. The levels of EL were tested by the method of enzyme linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). Results Smooth muscle cells were treated with increasing concentrations of IL-1β, ranging from 1 μg/L to 30 μg/L. The relative increases in EL protein and mRNA were greater at the highest concentrations (30 μg/L) of IL-1β. There was a significant difference between the intervention group and the control group (P<0.05 ). There were significant differences among the intervention groups (P<0.05). The relative increases in EL protein and mRNA were greater at the highest concentrations (50 μg/L) of TNF-α. There was a significant difference between the intervention group and the control group (P<0.05). There were significant differences among the intervention groups (P<0.05).Conclusion Smooth muscle cells were treated with increasing concentrations of IL-1β and TNF-α, then secreted protein and EL mRNA were noted.关键词
主动脉平滑肌细胞/内皮脂肪酶/白细胞介素1β/肿瘤坏死因子αKey words
Endothelial Lipase/Aortic Smooth Muscle Cells/Interleukin-1β/Tumor Necrosis Factor-α分类
医药卫生引用本文复制引用
杨晓静,成丽英,边云飞,赵欣,肖传实..白细胞介素1β和肿瘤坏死因子α对大鼠主动脉平滑肌细胞内皮脂肪酶表达的影响[J].中国动脉硬化杂志,2009,17(3):181-184,4.基金项目
山西医科大学第二医院院内基金项目(20070102) （20070102）
