解放军医学杂志2001，Vol.26Issue(3)：182-185,4.
抑制生物蝶呤合成对大鼠烫伤后金葡菌感染所致肺损伤的影响
THE EFFECT OF BIOPTERIN SYNTHESIS INHIBITOR ON ACUTE LUNG INJURY IN POSTBURN STAPHYLOCOCCUS AUREUS SEPSIS
李红云 1姚咏明 1施志国 1董宁 1于燕 1陆连荣 1盛志勇1
作者信息
摘要
Abstract
To determine the role of biopterin in acute lung injury in scald rats with infection, and to observe the therapeutic effect of GTP-cyclohydrolase I(GTP-CHI) inhibitor on postburn sepsis, 56 male Wistar rats were randomly divided into four groups: normal controls (n=10), scald control group(n=10), postburn sepsis group(n=20) and 2,4-diamino-6-hydroxy-pyrimidine (DAHP) treatment group (n=16). Tissue samples from lungs were collected to determine GTP-CHI and inducible nitric oxide synthase (iNOS) mRNA expression, meanwhile biopterin, nitric oxide (NO), tumor necrosis factor-α(TNF-α) and interferon-γ (IFN-γ) levels in lungs were also measured. The results showed that after scald injury followed by Staphylococcus aureus challenge, GTP-CHI gene expression and biopterin levels significantly elevated in lungs, so did the values of iNOS mRNA expression and NO induction (P<0.05～0.01). Pulmonary myeloperoxidase (MPO) activities were also increased. Early treatment with DAHP could significantly reduce GTP-CHI/biopterin induction as well as iNOS/NO levels and MPO activities. The levels of TNF-α and INF-γ both in tissue and plasma were also decreased to certain extent. These results suggest that excessive production of biopterin may play an important role in the patho-genesis of acute lung injury after severe burns combined with Staphylococcus aureus challenge, and early treatment with biopterin synthesis inhibtor might be a potential strategy to prevent the development of sepsis.关键词
烧伤/葡萄球菌,金黄色/生物蝶呤/一氧化氮/脓毒症分类
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李红云,姚咏明,施志国,董宁,于燕,陆连荣,盛志勇..抑制生物蝶呤合成对大鼠烫伤后金葡菌感染所致肺损伤的影响[J].解放军医学杂志,2001,26(3):182-185,4.
