首页|期刊导航|中华医学杂志（英文版）|Expression of ciliary neurotrophic factor after induction of ocular hypertension in the retina of rats
中华医学杂志（英文版）2007，Vol.120Issue(20)：1825-1829,5.
Expression of ciliary neurotrophic factor after induction of ocular hypertension in the retina of rats
Expression of ciliary neurotrophic factor after induction of ocular hypertension in the retina of rats
摘要
Abstract
Background Glaucoma is mainly characterized by the loss of retinal ganglion cells. Ciliary neurotrophic factor (CNTF) is believed to stimulate the regeneration of axons of retinal ganglion cells. The objective of our study was to detect the expression of CNTF in the retina of a rat glaucoma model with increased intraocular pressure (lOP).Methods The rat glaucoma model was set up by electrocoagulating at least three episcleral and limbal veins. The location and the expression level of CNTF were detected at 1, 3, 7, 14, and 28 days post-surgery by immunohistochemistry, semiquantitative reverse-transcription polymerase chain reaction (RT-PCR), and Western blot analysis.Results The rat glaucoma model with chronic, moderately elevated lOP was successfully produced. A minimum expression of CNTF was found in the ganglion cell layer of the retinas of the control group, and temporally increased expression and intensity of CNTF were found in the experimental retinas.Conclusion The expression of endogenous CNTF in the rat retina was found altered after the induction of ocular hypertension.关键词
ciliary neurotrophic factor/ ocular hypertension/ glaucoma/ Sprague-Dawley rats/ retinaKey words
ciliary neurotrophic factor/ ocular hypertension/ glaucoma/ Sprague-Dawley rats/ retina分类
医药卫生引用本文复制引用
WU Qiang,ZHANG Min,SONG Bei-wen,LU Bin,HU Ping..Expression of ciliary neurotrophic factor after induction of ocular hypertension in the retina of rats[J].中华医学杂志（英文版）,2007,120(20):1825-1829,5.基金项目
This study was supported by a grant from Shanghai Natural Science Foundation (No. 03ZR14067). （No. 03ZR14067）
