物理化学学报2007，Vol.23Issue(12)：1827-1830,4.
微流路中利用DNA选择性固定蛋白质
Selective Protein Immobilization via DNA Conjugation under Microfluidic Laminar Flow
摘要
Abstract
The feasibility of controlled protein immobilization via DNA conjugation by utilizing laminar flow in a microfluidic device was demonstrated. The glass surface in a microchannel was treated by oligonucleotides. The laminar flow brought different protein-DNA conjugates parallel into the microchannel. DNA recognition allows proteins to be delivered to the desired location. The total internal reflection fluorescence was also applied to monitor the process. Both the specificity and sensitivity were high, and the immobilization and removal of the proteins were repeatable. It was shown that with parallel detection of specific and non-specific recognitions, the accuracy of bioassay would be effectively enhanced. This strategy could improve the performing quality of biosensors in microfluidic devices.关键词
微流路/蛋白质传感器/DNA引导的固定/全内反射荧光Key words
Microfluidic device/ Protein sensor/, DNA-directed immobilization/ Total internal reflection fluorescence分类
化学化工引用本文复制引用
郭素,薛面起,钱民协,曹廷炳,赵新生..微流路中利用DNA选择性固定蛋白质[J].物理化学学报,2007,23(12):1827-1830,4.基金项目
国家重点基础研究发展专项经费(2006CB910304)及国家自然科学基金(20333010,20403001,20673002)资助 （2006CB910304）
