中国免疫学杂志2001，Vol.17Issue(4)：183-185,3.
白细胞介素12提高HBV基因疫苗的免疫应答
Interleukin 12 potentiates immune response induCed by DNA vaccine
杜德伟 1周永兴 1焦成松 1冯志华 1李谨革 1连建奇 1姚志强1
作者信息
摘要
Abstract
Objective:To observe the immune responses to gene vaccirnes coding HBV surface antigen and intedeukin 12 in Balb/c(H-2d ) mice.Methods:The immunization was performed by intramuscular injection in this experiment. Anti-HBs in serum was detected by ELISA.HBsAg specific cytotoxic T 1ymphocytes (CTLs) activity was measured by 51Cr release assay. Resiuits:8 w after immunization,the serum OD value of the mice coinjected IL-12 vaccine (1 .64± 0.15) is significantly higher than that of mice injected HBV-S DNA vaccine (0.87±0. 1)only. CTLs activity of the mice injjected HBV-S DNA vaccine and coinjected IL-12vaccine were 50.5%±6.4% and 73.3%±8.8% respec-tively. After adding anti-CD4+ monoclonal antihody in spleen cells, CTLs activity of the mice injected HBV-S DNA vaccine and coinjeted IL-12 vaccine were 48.3% ± 5.9% and 75.6% ± 9.1% respectively. CTLs activity were 10.6% ± 1.4% and 16.3% ± 2.3% respectively after adding anti-CD8+ monoclonal antibody in spleen cells.Conchusion:The results skowed that the DNA vaccine of pCR3. 1-S had strong antige-necity in cellular and humoral immunity which can be promoted by vector coding nurine IL-12. CTLs acivity were performed by CD8+ cells.DNA vaccine against HBV may be useful for both prophylactic and therapeutic purposes.关键词
基因疫苗乙型肝炎表面抗原IL-12真核表达载体分类
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杜德伟,周永兴,焦成松,冯志华,李谨革,连建奇,姚志强..白细胞介素12提高HBV基因疫苗的免疫应答[J].中国免疫学杂志,2001,17(4):183-185,3.
