中国当代儿科杂志2007，Vol.9Issue(3)：183-187,5.
11β-羟甾类脱氢酶抑制剂对高脂喂养SD大鼠体重及糖耐量的影响
Effects of 11β-hydroxysteroid dehydrogenase inhibitor on body weight and glucose tolerance in Sprague-Dawley rats fed with a high-fat diet
摘要
Abstract
Objective Many studies have shown that glucocorticoids play a crucial role in the development of obesity and insulin resistance. This study investigated the therapeutic effects of long-term inhibition of glucocorticoid activity on obesity and insulin resistance. Methods Four-week-old male Sprague-Dawley (SD) rats were randomly fed with a highfat diet ( fat content accounting for 20% of total calorie ) ( control group, n = 8 ) or with a high-fat diet along with glycyrrhetic acid (GE, 800 mg/L), an inhibitor of 11 β-hydroxysteroid dehydrogenase (11β-HSD) for 24 weeks (GEtreated group, n =9). The body weights and the amount of food intake were monitored weekly and daily, respectively. After 24 weeks of GE treatment, oral glucose tolerance tests were performed. Blood glucose was measured by glucose oxidase method. The levels of plasma glucocorticoids, insulin and leptin were measured with radioimmunoassay. The levels of serum cholesterol and triglyceride were determined with an automatic measuring analyzer. Results The food intake amount decreased significantly in the GE-treated group from 6 weeks and body weight gain was markedly less from 8 weeks after GE administration compared with the control group. After 24 weeks of treatment, the plasma levels of leptin and insulin in GE- treated rats were significantly reduced compared with the control group. The serum levels of cholesterol and triglyceride decreased markedly compared with the control group and the levels of blood glucose were significantly lower 15,30, 60 and 120 minutes after oral glucose load in the GE-treated group compared with the control group. Conclusions Long-term GE treatment may contribute to resisting diet-induced obesity and insulin resistance.关键词
11β-羟甾类脱氢酶抑制剂/糖皮质激素/肥胖症/糖耐量/大鼠Key words
11β-hydroxysteroid dehydrogenase inhibitor/Glucocorticoids/Obesity/Glucose tolerance/Rats分类
医药卫生引用本文复制引用
刘正娟,边杰,王玉川,闫冬,汪晓霞..11β-羟甾类脱氢酶抑制剂对高脂喂养SD大鼠体重及糖耐量的影响[J].中国当代儿科杂志,2007,9(3):183-187,5.基金项目
China Natural Scientific Foundation (No. 30571577). （No. 30571577）
