中国临床康复2006，Vol.10Issue(30)：184-186,3.
人bcl-xl转基因小鼠外源基因的复制及其传代稳定性
Reproduction and stable passage of foreign gene in human bcl-xl transgenic mice
摘要
Abstract
BACKGROUND: Recent studies indicate that bcl-xl gene may inhibit the apoptosis of neurons in cerebral ischemia and repeff usion injury to exert the brain protection function.OBJECTIVE: To develop human bcl-xl transgenic mice by microinjection and detect the reproductionof the foreign gene and the stability of the passage.DESIGN: A gene-based observation.SETTINGS: Department of Neurology, Tongji Hospital affiliated to Tongji Medical College, Huazhong University of Science and Technology; Hubei Academy of Agriculture and Forestry Sciences, Research Institute of Animal Husbandry and Medicine.MATERIALS: The experiment was carried out in the Tongji Hospital affiliated to Tongji Medical College, Huazhong University of Science and Technology. Four Kunming mice were used.METHODS: Human bcl-xl gene transgenic mice were established with microinjection. The positive mice with integration detected by polymerase chain reaction (PCR) and Southern-blot were copulated with normal mice and passaged, and then the heredity of foreign gene in the filial generation was detected. The results of Southern-blot assay showed that the mice of No. 10, 13, 6 and 5 were positive, and they were taken as the initial ones, copulated with the normal Kunming mice respectively, proliferated and passed to the filial generation, called generation F1. The F1 to F4 of each series were produced by such a way to keep them heterozygotes.MAIN OUTCOME MEASURES: The reproduction and stable transmissibility of foreign gene in the offspring were detected.RFSULTS: The PCR positive rate of No. 10 mouse kept stable, accorded with the Mendelian inheritance rule, and the foreign gene could inherit steadily. The PCR positive rate of No.6 mouse showed the tendency of a little descent, but could maintain the relatively stable inheritance. For No.13 and 5 mice, there were notable descent, indicating that the foreign gene in this mice family had been lost during the heredity.CONCLUSION: Transgenic mice line can be obtained by taking genome DNA as the aim gene.关键词
小鼠国转基因/基因/DNA复制分类
医药卫生引用本文复制引用
王芙蓉,袁慧,肖文伍,张苏明,赵浩斌,郑新民,魏庆信..人bcl-xl转基因小鼠外源基因的复制及其传代稳定性[J].中国临床康复,2006,10(30):184-186,3.基金项目
国家自然科学基金重点资助项目(39730170) （39730170）
国家自然科学基金面上项目(39770810) the Key Project of National Natural Science Foundation of China, No. 39730170 （39770810）
the General Program of National Natural Science Foundation of China, No. 39770810 （）
