中国临床康复2005，Vol.9Issue(34)：184-186,189,4.
聚乙烯醇/羟基磷灰石复合水凝胶移植修复兔膝关节软骨缺损
Experimental study of rabbit knee joint articular cartilage defects repaired with transplantation of polyvinyl alcohol/hydroxyapatite composite hydrogel
摘要
Abstract
BACKGROUND: At present, the therapy to treat articular cartilage lesions caused by trauma, osteoarthritis and other diseases fails to repair the damaged cartilage to its normal state morphologically, biochemically and biomechanically and for the repaired tissues, good long-term effect doesn't happen and retrograde degeneration happens in a short period.OBJECTIVE: To study the curative effect of transplantation of polyvinyl alcohol/hydroxyapatite (PVA/HA) composite hydrogel to repair rabbit knee joint articular cartilage defects and to learn about its biocompatibility with host tissues.DESIGN: A randomized controlled experimental animal study.SETTING: The Animal experimental Center of the Third Affiliated Hospital of Sun Yat-sen University.MATERIALS: The experiment was conducted at the Animal Experimental Center of the Third Affiliated Hospital of Sun Yat-san University. Totally 20 adult healthy New Zealand white rabbits including 12 males and 8 females, with a body mass of 2.1 to 3.0 kg were provided by the Animal Experimental Center of Sun Yat-sen University. All animals were bred in an environment of 20℃ and 40% of humidity. The rats were randomized into the blank control group (n=6) and the experimental group (n=14).METHODS: Polyvinyl alcohol/hydroxyapatite composite hydrogel was made by sol-gel method of hydroxyapatite powder. The tensility was tested at a pulling speed of 500 mm/min by AG-1 omnipotence electronic pulling tester (produced by Shimadz Co. of Japan) through mechanical property test in vitro. Rabbits in the control group received no other procedures while the rabbits in the experimental group had their knee joint articular cartilage defects repaired with transplantation of cylindrical PVA/HA composite hydrogel, fixed by inlaying the hydrogel into the defect. Fed in the cage alone postoperatively, all animals were permitted to move without any immobilization to the operated limbs. Routine therapy of benzylpenicillin and streptomycin anti-infection was performed. Then, 6-7 animals were sacrificed by the intravenous injection of air emboli 4, 8 and 12 weeks after the operation for examining the specimens from the operated knees macroscopically and microscopically.MAIN OUTCOME MEASURES: Primary results: macroscopic and histological evaluation of the articular cartilage defects of each group. Secondary results: The relationship between the tensile strength of PVA/HA composite hydrogel in vitro and its soaking time.fects of the rabbits in the two groups: 4 weeks after the operation, the defects in the experimental group were filled up with PVA/HA. No space was found between the material and the subchondral bone. 12 weeks after the operation. A lot of chondrocytes proliferated on the boundaries between the grafts and the normal cartilage and no cartilage degeneration took place.The graft's bottom was tied to the subchondral bone and some osteoid tissues had grown into it. Fiber granulation tissue repair mainly showed up in HA composite hydrogel in vitro and its soaking time: Tensile strength of PVA/HA increased in the early soaking time (in 6 days), then decreased a bit, and then became stable henceforth. But the eventual tensile strength was higher than it was at the beginning.CONCLUSION: PVA/HA composite hydrogel was well combined with subchondral bone without inflammatory cell infiltration and immunological rejection, suggesting a good biocompatibility with host tissues. After the implantation, there was no cartilage cellular degeneration, suggesting that there was certain mechanical effect. This indicates that PVA/HA can be used as a good substitute material for artificial cartilage. Polyvinyl alcohol / hydroxyapatite composite hydrogel was adopted by sol-gel in situ method of hydroxyapatite powder to avoid the degradation and exfoliation of coat materials and to keep good mechanical characteristics for a long time.关键词
软骨,关节/生物相容性材料/羟基磷灰石类/移植分类
医药卫生引用本文复制引用
卢华定,蔡道章,刘青,王迎军,郑裕东..聚乙烯醇/羟基磷灰石复合水凝胶移植修复兔膝关节软骨缺损[J].中国临床康复,2005,9(34):184-186,189,4.基金项目
广东省医学科研项目(B2002040)资助Medical Scientific Research Program of Guangdong Province, China, No. B2002040 （B2002040）
