中德临床肿瘤学杂志（英文版）2006，Vol.5Issue(3)：184-188,5.
中国非小细胞肺癌患者血清DAPK基因、p16基因启动子甲基化的分析
The Promoter Hypermethylation of DAPK Gene and p16 Gene in Sera from Chinese Non-small Cell Lung Cancer Patients
摘要
Abstract
Objective: To evaluate the clinical significance of the aberrant methylation of DAPK gene and p16 gene in sera from 65 NSCLC patients from Nanjing General Hospital of Nanjing Command, China.Methods: A methylation-specific PCR (MSP) was performed for the detection of promoter hypermethylation of DAPK gene and p16 gene in blood DNA from 65 cases of NSCLC, and to analyze the relation of the aberrant methylation of DAPK gene and p16 gene and the clinicopathological data. Results: 30.8%(20/65) of the sera from 65 cases of NSCLC showed hypermethylation for DAPK promoter and 43.1%(28/65) the same for p16 promoter, whereas no methylated DAPK gene promoter and p16 gene promoter were found in sera from the patients with lung benign diseases and normal controls. Methylated DAPK gene promoter and p16 gene promoter in sera were not closely correlated with the pathological classification, stage, metastasis and differentiation in NSCLC. Conclusion: Detection of the aberrant methylation of DAPK gene and p16 gene in blood DNA from NSCLC patients might offer an effective means for the earlier auxiliary diagnosis of the malignancy.关键词
非小细胞肺癌/DAPK基因/p16基因/血清/DNA甲基化Key words
NSCLC/DAPK gene/p16 gene/serum/DNA methylation分类
医药卫生引用本文复制引用
林勍,陈龙邦,唐永明,王晶..中国非小细胞肺癌患者血清DAPK基因、p16基因启动子甲基化的分析[J].中德临床肿瘤学杂志（英文版）,2006,5(3):184-188,5.基金项目
This project was supported by grants from the Scientific Research Foundation of Nanjing General Hospital of Nanjing Command (No. 2003017). （No. 2003017）
