首页|期刊导航|中国海洋湖沼学报（英文版）|Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber Apostichopus japonicus
中国海洋湖沼学报（英文版）2007，Vol.25Issue(2)：184-190,7.
Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber Apostichopus japonicus
Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber Apostichopus japonicus
摘要
Abstract
The manipulation of the chromosome set for commercially valuable marine animals is important for enhancing aquacultural production. In this study, triploid and tetraploid sea cucumber Apostichopus japonicus were induced by hydrostatic pressure shock, and the conditions of appropriate induction were tested with different starting times, and hydrostatic pressure intensities and durations. The highest rate of triploid induction reached 20% and that of tetraploid was 60%. In consideration of the survival rate and hatch rate, the appropriate treatment for triploid was 55 Mpa of hydrostatic pressure for 5 min at 55 min after fertilization (a.f.), while for tetraploid it was 60 Mpa for 5 min at 61 min a.f. The triploid of the sea cucumber could survive through the pelagic larval stage and attachment stage, and develop like the control group of the experiment. The tetraploid, however, could not survive the attachment stage.关键词
triploid/tetraploid/hydrostatic pressure shock/sea cucumber/Apostichopus japonicusKey words
triploid/tetraploid/hydrostatic pressure shock/sea cucumber/Apostichopus japonicus分类
生物科学引用本文复制引用
DING Jun,CHANG Yaqing,WANG Zichen,SONG Jian..Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber Apostichopus japonicus[J].中国海洋湖沼学报（英文版）,2007,25(2):184-190,7.基金项目
Supported by the National High-Tech Research and Development Program of China (863 Program, No.2506AA10A411), and by the Dalian Natural Science Foundation (No. 99058). （863 Program, No.2506AA10A411）
