北京大学学报（自然科学版）2001，Vol.37Issue(2)：186-191,6.
表面等离子体共振免疫传感的放大检测研究
Studies on Amplified Assay of Surface Plasmon Resonance Immunosensing
摘要
Abstract
Surface plasmon resonance(SPR)immunosensor is a relatively new immunoassay technique and has been receiving more and more attention in recent years.However,a major disadvantage of SPR for bioanalytical applications is that low concentration or low molecular mass analytes could not be detected directly.Therefore,it is a challenging task to develop strategies for improving the detection limit/sensitivity of SPR.In this paper,authors present a novel strategy for improving the sensitivity of SPR immunosensing using streptavidin-biotinylated antibody complex.It is proven that the amplification strategy causes a dramatic improvement of the detection sensitivity.This amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein.The complex can be formed in a cross-linking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex.关键词
表面等离子体共振技术/免疫传感器/放大检测/链霉亲和素-生物素化抗体复合物分类
化学化工引用本文复制引用
裴仁军,汪尔康,杨秀荣..表面等离子体共振免疫传感的放大检测研究[J].北京大学学报（自然科学版）,2001,37(2):186-191,6.基金项目
中国博士后科学基金和中国科学院王宽诚博士后工作奖励基金资助项目 （）
