南京医科大学学报（英文版）2003，Vol.17Issue(4)：187-190,4.
神经生长因子对大鼠脊髓损伤后N-甲基-D-天门冬氨酸受体1和神经元一氧化氮生成的影响
Effects of Nerve Growth Factor on NMDA Receptor 1 and Neuronal Nitric Oxide Production after Spinal Cord Injury in Rats
摘要
Abstract
Objective:To explore the protective mechanisms of nerve growth factor ( NGF)on spinal cord injury (SCI) and provide theoretical basis for its clinical application. Methods: The SCI of Wistar rats was done by Allen's weight dropping way by a 10 g × 2.5 cm impact on the posteri-or of spinal cord T8. NGF (3 g/L, 20 μl) or normal saline was injected through catheter into sub-arachnoid space 2, 4, 8, 12 and 24 h after SCI. The expression of N-methyl- D-asparate receptor1 (NMDAR 1 ) and neuronal constitutive nitric oxide synthase (ncNOS) mRNA in rat spinal cord was detected by in situ hybridization. Results: Abnormal expression of NMDAR 1 and ncNOS mRNA appeared in spinal ventral horn motorneuron in injured rats, as compared with that in control group. The expression of NMDAR 1 and ncNOS mRNA in NGF group was significantly lower than that in saline group (P＜0.01). Conclusion: NGF can protect spinal cord against injury in vivo. One of the mechanisms is that NGF can prohibit NMDAR 1 and nitric oxide (NO) production after spinal cord injury.关键词
神经生长因子/脊髓损伤/N-甲基-D-天门冬氨酸受体1/一氧化氮Key words
nerve growth factor/spinal cord injury/N-methyl-D-asparate recep-tor 1/nitric oxide分类
医药卫生引用本文复制引用
汤长华,曹晓建,王道新..神经生长因子对大鼠脊髓损伤后N-甲基-D-天门冬氨酸受体1和神经元一氧化氮生成的影响[J].南京医科大学学报（英文版）,2003,17(4):187-190,4.基金项目
Supported by a grant from NationalNatural Science Foundation of China (39800176) （39800176）
