中华医学杂志（英文版）2002，Vol.115Issue(12)：1873-1876,4.
低强度微波辐射对兔眼晶体上皮细胞间隙连接通讯功能的影响
Changes in gap junctional intercellular communication in rabbits lens epithelial cells induced by low power density microwave radiation
叶娟 1姚克 1曾群力 2鲁德强2
作者信息
- 1. 浙江大学医学院附属第二医院眼科中心,杭州,310009
- 2. 浙江大学生物电磁学研究所,杭州,310006
- 折叠
摘要
Abstract
Objective To demonstrate the changes in gap junctional intercellular communication (GJIC) mediated by low power density microwave radiation in rabbits lens epithelial cells (LECs) and its mechanisms.Methods Rabbits' eyes were exposed to 5 mW/cm2 and 10 mW/cm2 power densities of microwave radiation for 3 hours. The fluorescence-recovery-after-photobleaching (FRAP) method was used to determine the GJIC. The localization and function of connexin 43 in LECs was detected by laser scanning confocal microscopy.Results The GJIC of rabbits LECs was inhibited by microwave radiation especially in the 10 mW/cm2 irradiated samples. A decrease in connexin 43-positive staining was seen in 5 mW/cm2×3 h treated LECs. Intracellular space accumulation and cytoplasmic internalization were clearly demonstrated in 10 mW/cm2 group.Conclusions Low power densities microwave radiation (5 mW/cm2 and 10 mW/cm2) induces damage to connexin 43 and inhibits the GJIC of rabbits LECs. These changes result in an osmotic imbalance within the lens and induce early cataract. 5 mW/cm2 or 10 mW/cm2 microwave radiation is cataractogenic.关键词
微波辐射/晶状体上皮细胞/间隙连接/连接蛋白43/白内障Key words
microwave radiation * lens epithelial cells * gap junction * connexin 43 * cataractogenesis分类
医药卫生引用本文复制引用
叶娟,姚克,曾群力,鲁德强..低强度微波辐射对兔眼晶体上皮细胞间隙连接通讯功能的影响[J].中华医学杂志（英文版）,2002,115(12):1873-1876,4.
