中华医学杂志（英文版）2009，Vol.122Issue(2)：188-192,5.DOI:10.3760/cma.j.issn.0366-6999.2009.02.015
Celecoxib plays a multiple role to peripheral blood lymphocytes and allografts in acute rejection in rats after cardiac transplantation
Celecoxib plays a multiple role to peripheral blood lymphocytes and allografts in acute rejection in rats after cardiac transplantation
ZHANG Xue-feng 1ZHANG Fan 1LIU Hong-yu 1SUN Guo-dong 1LIU Zong-hong 1L(U) Hang 1CHI Chao 1LI Chun-yu1
作者信息
- 1. Department of Cardiac Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China
- 折叠
摘要
Abstract
Background Celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, is a non-steroidal anti-inflammatory drug used as an adjuvant to sensitize cancer cells to apoptosis. However, in rats suffering from acute rejection, celecoxib reduced apoptosis of myocardial cells. We hypothesize that celecoxib reduces myocardial apoptosis either by inducing apoptosis in peripheral blood lymphocytes (PBLs) or by altering the percentage of CD4+ and CD8+ lymphocytes. Methods After cardiac transplantation, rats were administered intragastrically with celecoxib (50 mg/kg per day) for 3, 5 or 7 days, at which time the graft was excised and evaluated for organ rejection. In addition, PBLs were isolated from the blood to determine PBLs apoptosis, and the percentage of CD4+ and CD8+ lymphocytes. Results Celecoxib induced PBLs apoptosis in 3 days, but protected the cells from apoptosis at 5 and 7 days. Also, the percentage of CD4+ lymphocytes decreased only at 3 days, but a reduction in the percentage of CD8+ lymphocytes was not seen until 7 days after the transplant surgery. Celecoxib only decreased acute rejection at 5 days, with no discernible difference in rejection after 3 and 7 days. Conclusions The results suggested that celecoxib displayed a multiple physiological function in a time-dependent manner.关键词
celecoxib/mitochondria/apoptosis/transplantation, heartKey words
celecoxib/mitochondria/apoptosis/transplantation, heart分类
医药卫生引用本文复制引用
ZHANG Xue-feng,ZHANG Fan,LIU Hong-yu,SUN Guo-dong,LIU Zong-hong,L(U) Hang,CHI Chao,LI Chun-yu..Celecoxib plays a multiple role to peripheral blood lymphocytes and allografts in acute rejection in rats after cardiac transplantation[J].中华医学杂志（英文版）,2009,122(2):188-192,5.
