中国动脉硬化杂志2009，Vol.17Issue(3)：189-192,4.
体外培养成人脂肪间充质干细胞及向平滑肌细胞诱导分化
Biological Characteristics of Adult Adipose-Derived Mesenchymal Stem Cells and Their Differentiation into Smooth Muscle Cells in Vitro
郭泽君 1边云飞 2薛君 1武卫东 2肖传实2
作者信息
- 1. 山西医科大学第二临床医学院,山西省太原市,030001
- 2. 山西医科大学第二医院心内科,山西省太原市,030001
- 折叠
摘要
Abstract
Aim To study the basic biological characteristics of adult adipose-derived mesenchymal stem cell (ADMSC) and the potential of differentiating into smooth muscle cells by chemical induction. Methods ADMSC were isolated and cultured from adult greater omentum adipose tissue in vitro. The morphological characteristics of cells were observed by the inverted microscope. The cell cycle and expression of CD29, CD31, CD34 and CD44 were detected by flow cytometry. The fifth passage cells were induced with 5 μg/L transforming growth factor-β(TGF-β) to differentiate into smooth muscle cells. Then the immunohistochemistry method was used to identify smooth muscle cells. Results ①The isolated and cultured ADMSC presented even fusiform shape and stably passaged, during which ADMSC kept the same shape. ②ADMSC expressed CD29 and CD44 but not CD34 or CD31 by flow cytometry. ③ The cell cycle studies re-vealed that there were 90.14% of the cells in G0/G1, 3.77% in S and 6.09% in G2/M. ④ After 10 days induction by TGF-β,the cells assumed a spindle shape and a "hill-and-valley" growth pattern. ⑤The induced cells expressed charac-teristic α-smooth muscle actin(α-SMA ) by immunohistochemistry. Conclusion Adult adipose tissues contain plenty of productive mesenchymal stem cells that can be easily isolated, cultured and differentiated into smooth muscle cells in vitro.关键词
人脂肪间充质干细胞/转化生长因子β/平滑肌细胞/细胞分化Key words
Adult Adipose-Derived Mesenchymal Stem Cells/Transforming Growth Factor-β/Smooth Muscle Cells/Cell Differentiation分类
医药卫生引用本文复制引用
郭泽君,边云飞,薛君,武卫东,肖传实..体外培养成人脂肪间充质干细胞及向平滑肌细胞诱导分化[J].中国动脉硬化杂志,2009,17(3):189-192,4.
