中国林学(英文版)2008，Vol.10Issue(3)：189-192,4.
Methodological comparison of DNA extraction from Holcocerrus hippophaecolus (Lepidoptera: Cossidae) for AFLP analysis
Methodological comparison of DNA extraction from Holcocerrus hippophaecolus (Lepidoptera: Cossidae) for AFLP analysis
摘要
Abstract
Amplified fragment length polymorphism (AFLP) is a powerful DNA fingerprinting technique for studying genetic rela-tionships and genetic diversity in insects. However, the crucial prerequisite for AFLP analysis is to extract DNA of high quality. In this study, we evaluate four different protocols (SDS method, improved SDS method, CTAB method and a complex method with SDS and CTAB) for isolating DNA from the seabuckthorn carpenter moth (Holcocerrus hippophaecolus (Lepidoptera: Cossidae)). The results indicate that the CTAB method does not produce DNA suitable for AFLP analysis. The SDS method and the complex method with SDS and CTAB are comparatively time-consuming and resulted in low yields of DNA and were therefore not used for AFLP assay. The improved SDS method is recommended for preparing DNA templates from H. hippophaecolus for AFLP analysis.关键词
DNA extraction/Holcocerrus hippophaecolus/AFLPKey words
DNA extraction/Holcocerrus hippophaecolus/AFLP分类
农业科技引用本文复制引用
CHEN Min,ZHU Yang-yu,TAO Jing,Luo You-qing..Methodological comparison of DNA extraction from Holcocerrus hippophaecolus (Lepidoptera: Cossidae) for AFLP analysis[J].中国林学(英文版),2008,10(3):189-192,4.基金项目
This work was supported by the program for Changjiang Scholars and Innovative Research Team in Universties (PCSIRT0607)and by the jointly-funded project of the Beijing Municipal Education Commission Research Base. （PCSIRT0607）
