华北农学报2005，Vol.20Issue(5)：1-9,9.
通过基因枪和农杆菌介导用BADH基因转化小麦
Transformation of Wheat with BADH Through Gene Gun and Agrobacterium
摘要
Abstract
Salinity is very severe environmental problem hampering crop production. BADH is an important gene for conferring osmotic stress tolerance to plants. In this study gene gun and Agrobacterium were employed for the delivery of BADH into callus of wheat. Bombardment of 2 933 calli derived from immature embryos resulted in regeneration of 45plant-lets after selection on phosphinothricin (PPT) with net regeneration rate of 1.53 %. PCR analysis confirmed the presence of BADH gene in five plants. Results of PPT-leaf painting conformed to PCR test. In case of Agrobacterium mediated transformation five plant-lets regenerated from inoculation of 1 968 calli derived from mature embryos but none was PCR positive. PCR test of calli from mature embryos exhibited the successful delivery and integration of transgene. Inoculation of 2 933 calli from immature embryos resulted in regeneration of 21 plant-lets. PCR evaluation of regenerated plantlets indicated the presence of BADH in 5 plants only. Net regeneration rate for immature embryos (0.72%) was higher than that of mature embryos (0.25 % ). Transformed plants obtained through Agrobacterium and gene gun were able to thrive in 0.5 % NaCl (w/w) without stunted growth and wilting as compared with check plants indicating the successful integration and expression of BADH.关键词
遗传转化/小麦/农杆菌/基因枪/BADH基因Key words
Transformation/Wheat/Agrobacterium/Gene gun/BADH分类
农业科技引用本文复制引用
RAZZAQ Abdul,张艳敏,赵和,马峙英,郭北海,王海波..通过基因枪和农杆菌介导用BADH基因转化小麦[J].华北农学报,2005,20(5):1-9,9.基金项目
河北省自然基金项目(303606) （303606）
