首页|期刊导航|中华医学杂志（英文版）|Effect of antimicrobial agents on the toll-like receptors and inflammatory cytokines in liver tissue of the alcohol-induced liver disease in rats with Vibrio vulnificus sepsis
Effect of antimicrobial agents on the toll-like receptors and inflammatory cytokines in liver tissue of the alcohol-induced liver disease in rats with Vibrio vulnificus sepsis
LU Zhong-qiu LI Meng-fang QIU Qiao-meng LIANG Huang ZHOU Tie-li HONG Guang-liang WU Bin
中华医学杂志（英文版）2009，Vol.122Issue(16)：1910-1916,7.
中华医学杂志（英文版）2009，Vol.122Issue(16)：1910-1916,7.DOI:10.3760/cma.j.issn.0366-6999.2009.16.014
Effect of antimicrobial agents on the toll-like receptors and inflammatory cytokines in liver tissue of the alcohol-induced liver disease in rats with Vibrio vulnificus sepsis
Effect of antimicrobial agents on the toll-like receptors and inflammatory cytokines in liver tissue of the alcohol-induced liver disease in rats with Vibrio vulnificus sepsis
摘要
Abstract
Background Septicemia and inflammation-mediated septic shock caused by Vibrio vulnificus (VV) is strongly associated with chronic liver disease.This study examined the effects of antimicrobial therapy on expression of hepatic toll-like receptors and inflammatory cytokines in rats with alcohol-induced liver disease complicated by VV sepsis.Methods Male Sprague-Dawley rats were assigned to the following treatment groups:normal control (N),alcoholic liver disease control (A),antimicrob;al-treated alcoholic liver disease control (AA),alcoholic liver disease with VV sepsis (AV),and antimicrobial-treated alcoholic liver disease with VV sepsis (AVA).Alcohol-induced liver disease was observed in all groups except N.Expression of mRNAs encoding hepatic toll-like receptors 2 and 4,myeloid differentiation protein-2,tumor necrosis factor-α (TNF-α),interleukin (IL)-1β,IL-β and IL-10 was determined by RT-PCR.Results mRNAs encoding toll-like receptors 2 and 4 and myeloid differentiation protein-2 were significantly up-regulated in group AV as compared to control groups at 2-24 hours of sepsis;peak expression occurred at 12 hours.These mRNAs were also up-regulated in group AVA but to lesser degrees than in group AV at comparable time post-infection,mRNAs encoding TNF-α,IL-1β and IL-6 were significantly elevated in group AV as a function of infection.In group AVA as compared to AV,expression of TNF-α and IL-1β mRNAs was lower at 12-24 hours post-infection and expression of IL-6 mRNA was lower at 24 hours post-infection.Compared with control groups,IL-10 mRNA expression in group AV was markedly higher at 12-24 hours of sepsis.Expression of IL-10 mRNA was lower in group AVA as compared to AV at 24 hours of sepsis.Conclusions Antimicrobial therapy reduces expression of toll-like receptors and cytokines in rats with alcohol-induced liver disease complicated by VV sepsis.Monitoring hepatic toll-like receptor and cytokine expression during antibiotic therapy may be valuable for determining the course of VV sepsis in subjects with liver disease.关键词
Vibrio vulnificus/sepsis/toll-like receptors/inflammatory cytokines/cefoperazone sodium/levofloxacinKey words
Vibrio vulnificus/sepsis/toll-like receptors/inflammatory cytokines/cefoperazone sodium/levofloxacin分类
医药卫生引用本文复制引用
LU Zhong-qiu,LI Meng-fang,QIU Qiao-meng,LIANG Huang,ZHOU Tie-li,HONG Guang-liang,WU Bin..Effect of antimicrobial agents on the toll-like receptors and inflammatory cytokines in liver tissue of the alcohol-induced liver disease in rats with Vibrio vulnificus sepsis[J].中华医学杂志（英文版）,2009,122(16):1910-1916,7.基金项目
This study was supported by a grant from the Zhejiang Medical Key Assistance Programme (No.07-F04),Zhejiang Technology Project (No.2005C30009) and Key Project of Wenzhou Technology Bureau (No.Y2004A004). （No.07-F04）
