中国现代医学杂志2007，Vol.17Issue(16)：1921-1926,1930,7.
卡托普利对高氧致新生大鼠慢性肺疾病的保护作用及机制探讨
Effects of Captopril on fibrosis in lung tissue of neonatal rats with CLD Induced by hyperoxia
摘要
Abstract
[Objective] To observe the dynamic changes and the effects of rennin-angiotensin system and Captopril on interstitial fibrosis in lung of neonatal rats with CLD induced by hyperoxia. [Methods] 240 term neonatal Wistar rats were randomly assigned into air control, model, normal saline control and Captopril-treated groups (n=60each). The air control group was exposed to air ( FiO2=0.21 ). The rest three groups were continuously exposed to hyadministration. The Normal saline control group was administrated with normal saline instead. The model group did not have treatment. At the 1st, 3rd, 7th, 14th day and 21st days of exposure, the subjects were sacrificed. The changes of Angiotesin Converting Enzyme(ACE) , Angiotensin Ⅱ (Ang Ⅱ ), type Ⅰ collagen and mRNA expression were measured by enzyme-linked immunosorbentassay, radio-immunity technique and RT-PCR. The changes of lung histomorphology were observed. [Results] The ACE, Ang Ⅱ ,Type Ⅰ Collagen levels and mRNA expression increased significantly in the model and normal saline control groups on the 14th and 21st days of exposure compared with those of the air control group (P ＜0.05, or P ＜0.01). Captopril treatment reduced significantly the level and the expression of ACE, AngⅡ,Type Ⅰ Collagen, and there was no significant difference on days 1st, 3rd, 7th in the model group and normal saline control group compared with those of the air control group(P ＞0.05). But there were significant differences in the three results between the Captopril-treated and air control groups. The histopathological examination demonstrated different degrees of alveolitis, broaden interstitium and reduced alveolar quantity in the model group and normal saline control group compared with control group. [Conclusion] The changes of ACE, Ang Ⅱ, type Ⅰ Collagen levels and mRNA expression were increased signifcantly on the 14th and 21st days in lung of neonatal rats with CLD induced by hyperoxia. The histopathological examination demonstrated different degrees of alveolitis, broaden interstitium and reduced alveolar quantity in the CLD induced by hyperoxia; Captopril may have protective effects on lung injury induced by hyperoxia.关键词
新生大鼠/高氧/纤维化/肾素-血管紧张素系统/卡托普利Key words
rat/hyperoxia/fibrosis/rennin-angiotensin system/Captopril分类
医药卫生引用本文复制引用
李玖军,薛辛东..卡托普利对高氧致新生大鼠慢性肺疾病的保护作用及机制探讨[J].中国现代医学杂志,2007,17(16):1921-1926,1930,7.基金项目
Fund from National natural science ( No:30440056) （ No:30440056）
