中国现代医学杂志2006，Vol.16Issue(13)：1929-1932,1940,5.
BV2对MSC接受受损的PC12上清刺激后神经营养功能影响的研究
Effect of BV2 on neurotrophic activity of MSC stimulated with supernatant of injured PC12
摘要
Abstract
[Objective] To study whether and how microglias (BV2) affect the neurotrophic acitvity of marrow stromal cells (MSC) after being stimulated with injured PC12 media. [Methods] Primary culture was adopted for MSC culture, BV2 and PC12 were cultured as substitutes for microglias and neurons, respectively. Flow Cytometry was used to detect PC12 apoptosis, and ELISA for neurotrophic factor assay in MSC supernatant. [Results] With BV coculture, the PC12 apoptosis was reduced to the least when incubated with injured PC12 media. [Conclusion]Microglias are helpful in the neurotrophic activity of MSC stimulated with supernatant of injured neurons.关键词
小胶质细胞/骨髓基质细胞/神经营养Key words
microglias/marrow stromal cell/neurotrophic分类
医药卫生引用本文复制引用
罗晓光,吴晓芝,葛春林,周进,闫荣,张朝东..BV2对MSC接受受损的PC12上清刺激后神经营养功能影响的研究[J].中国现代医学杂志,2006,16(13):1929-1932,1940,5.基金项目
Supported by Scientific Research Project for higher education of Liaoning Province (No. 051522) （No. 051522）
