中国现代医学杂志2005，Vol.15Issue(13)：1930-1934,5.
罗丹明123及P-糖蛋白在人支气管损伤修复过程中的表达
Study of Rhodamine 123 staining and P-glycoprotein expression of human bronchial epithelium during wound-repair process induced by fluorouracil
摘要
Abstract
[Objective] To explore the condition of Rhodamine123 (Rh123) staining and the expression of P-glycoprotein(P-gp) of human bronchial epithelium in vitro during the wound-repair process induced by fluorouracil(5-FU). [Methods] The normal and 5-FU injurious bronchial epithelial cells were obtained by enzymatic digestion and stained with Rh123, then analyzed by flow cytometry. The expression of P-gp in normal and injurious groups were detected by immunofluorescence and western-blotting. Reverse transcriptase polymerase chain reaction (RT-PCR)was applied to detection of P-gp mRNA. [Results] The percentage of Rh123 negative staining live cells in injurious group was higher than normal group. With the wound-repair process, the percentage of Rh123 negative live cells gradually fall to nearly normal level. The expression of P-gp could be seen on the smear of bronchial epithelium in injurious group by immunofluorescence. The expression levels of P-gp and its mRNA were all higher after 5-FU treatment than the normal group. [Conclusions] There are bronchial stem cells which have the capacity to efflux Rh123 and express P-gp after 5-FU treatment. The proliferation and differentiation of these stem cells can regenerate bronchial epithelium.关键词
支气管/干细胞/氟尿嘧啶/人/罗丹明123/P-糖蛋白Key words
bronchial epithelium/stem cells/fluorouracil/human/rhodamine123/P-glycoprotein分类
医药卫生引用本文复制引用
周莹,贾心善..罗丹明123及P-糖蛋白在人支气管损伤修复过程中的表达[J].中国现代医学杂志,2005,15(13):1930-1934,5.基金项目
Supported bv the National Natural Science Foundation of China (No: 30170407) （No: 30170407）
