华西医科大学学报2001，Vol.32Issue(2)：194-195,207,3.
外源性Rb基因转染对头颈肿瘤的抑制作用
Adenovirus-mediated Rb Gene Transfect for Head and Neck Cancer
张晓晴 1刘世喜 2梁传余 2杨焕2
作者信息
- 1. 华西医科大学附属第四医院耳鼻咽喉科,
- 2. 附属第一医院耳鼻咽喉科
- 折叠
摘要
Abstract
Objective To evaluate the efficacy of Rb gene therapy in head and neck carcinoma. Methods We investigated the adenovirus-mediated Rb (Ad-Rb) gene transfect into human oral squamous cell carcinoma cell line (012) in both in vitro and in vivo models. Results The cells growth curve for 012 tumor cells transfect with Ad-Rb became lower after two days transfection and declined slightly by six days. Tumor growth in nude mice was also significantly retarded following Ad-Rb injection when compared with controls. Ad-Rb expression was identified by Western blotting in those tumors injected with Ad-Rb. Conclusion These results implicated that the recombinant Rb gene adenovirus vector could efficiently transfect and inhibit oral squmous cell carcinoma. It might be an important candidate for gene therapy in head and neck cancer.关键词
腺病毒载体 Rb基因 口腔鳞状细胞癌分类
医药卫生引用本文复制引用
张晓晴,刘世喜,梁传余,杨焕..外源性Rb基因转染对头颈肿瘤的抑制作用[J].华西医科大学学报,2001,32(2):194-195,207,3.
