中国临床康复2004，Vol.8Issue(10)：1949-1951,3.
大鼠脑缺血再灌注时小胶质细胞活化与外源性巨噬细胞补充
Activation of microglia and recruitment of extraneous macrophage following ischemia-reperfusion in adult rats
袁琼兰 1邓莉 1高小青 1李瑞祥 2羊惠君 2张光鹏2
作者信息
- 1. 泸州医学院神经生物学研究室,四川省,泸州市,646000
- 2. 四川大学华西医学中心解剖学教研室,四川省,成都市,610041
- 折叠
摘要
Abstract
AIM: To study the activation and proliferation of microglia/macrophages in cerebral tissue in rats following ischemia-reperfusion.METHODS: The ischemia model was established by blocking middle cerebral artery for 2 hours and reperfusing for 0. 5 -48 hours, and control sham operated(n=3) and normal(n=3) rats were processed. The immunocytochemical and lectin GSI-B4 by single and double staining were used to investigate temporal profile and morphological characteristics of microglia/macrophages after induction of focal cerebral ischemia in adult rats.RESULTS: There were many GSI-B4-positive microglias/macrophages with different morphologies as early as within 0. 5 hour. The number of positive cell declined from 3 to 6 hours and increased again from 12 to 48hours. The GSI-B4-positive macrophages were located in the superficial layer of preoptic area and cortical amygdaloid nucleus and meninges covering them. In control groups and all ischemia-reperfusion groups, PCNA positive immunohistochemistry was present in the ventricular and subventricular zones(VZ/SVZ) .Moreover, the strong PCNAimmunoreactivityandGSI-B4 strong positive amoebic-like macrophages were present in amygdaloid nucleus,cortical amygdaloid nucleus and meninges covering them. The double staining for PCNA and CD45RB existed the same cell from 24 to 48 hours.CONCLUSION: In ischemia-reperfusion, the microglia showed distinctly different morphologies. The extraneous macrophages may deride from not only the proliferation, immigration of macrophages in meninges, but also from the proliferation, immigration and differentiation of VZ/SVZ.关键词
再灌注损伤/巨噬细胞/大鼠分类
医药卫生引用本文复制引用
袁琼兰,邓莉,高小青,李瑞祥,羊惠君,张光鹏..大鼠脑缺血再灌注时小胶质细胞活化与外源性巨噬细胞补充[J].中国临床康复,2004,8(10):1949-1951,3.
