中国免疫学杂志2001，Vol.17Issue(4)：195-197,3.
McAb共刺激PBLs膜表面分子表达与肝癌细胞凋亡的相关性
Correlation of McAb costimulation PBLs membrane surface molecular expression and hepatoma cell apoptosis
摘要
Abstract
objective:To study phenotype changes in PBLs costimulated by McAb and correlation of phenotype changes and apoptosias in hepatoma cell.Methods:Cell phenotype changes in PBLs were analyzed of flow cytometry.By mean of hypermicroscopic structure and DNA ladder electrophoresis,hepatoma cell apoptosis was studied.Results:Expression of CD3、CD4 in stimulated PBLs was about 10 percent more than those eithout stimulation Nucleus pyknotic、border gathering and collapse were found in hepatoma cell, which showed typical apoptosis.DNA ladder appeared in electrophoresis of hepatoma DNA.CD3 and CD8 increased in tumor patients PBLs being co-stimulated(P<0.05).Conclusion:Co-stimulatng signal molecular increased,presenting antigen enhanced and cytokine secreted more by co-stumulating. Hepatoma cell apoptosis was bringed by these factors. T cell of tumor patientiated to CD8+ . Correlation of membrane molecular expression and apoptosis was showed by the results.关键词
单抗共刺激膜分子肝癌细胞凋亡分类
医药卫生引用本文复制引用
明利华,陈维春,林月霞,黄树林,符生苗,邓立群..McAb共刺激PBLs膜表面分子表达与肝癌细胞凋亡的相关性[J].中国免疫学杂志,2001,17(4):195-197,3.基金项目
国家自然科学基金资助 （）
