广东医学2001，Vol.22Issue(3)：195-198,4.
逆转录病毒介导的HSV-TK基因对
人骨肉瘤细胞的杀伤作用
Study on killing effect of retrovirus-mediated HSV-TK gene therapy for osteosarcoma
摘要
Abstract
Objective To study the gene therapy for osteosarcoma in vitro.Methods Retroviral vector and HSV-TK/GCV system were used.Results Recombinant retroviral vector (DORHyTK) containing Hygromycin phosphotransferase-thymidine Kinase fusion gene(HyTK) was constructed and introduced into PA317 retrovirus packaging cell by DOTAP. After selective culturing and measuring of retroviral titers, the osteosarcoma cells OS732 and SAOS-2 were infected by pseudotype retroviral supernatant, which did not contain replication-competent retrovirus(RCR). The HSVTK gene existence and expression in OS732TK and SAOS-2TK cells were examined by PCR and RNA dot blot analysis. HSV-TK/GCV gene therapy system showed its strong killing effects in osteosarcoma cells OS732 and SAOS-2 by means of MTT colorimetric assay, with the former stronger than the latter. However the “bystander effect” was weeker in OS732 than in SAOS-2 and was stronger in the high-density population group. Conclusion HSV-TK/GCV gene therapy system may provide a new therapeutic approach for treatment of osteosarcoma.关键词
骨肿瘤/基因治疗/单纯疱疹病毒胸苷激酶-丙氧鸟苷分类
医药卫生引用本文复制引用
乔慧,丘钜世,朱全胜..逆转录病毒介导的HSV-TK基因对
人骨肉瘤细胞的杀伤作用[J].广东医学,2001,22(3):195-198,4.
