作物学报2000，Vol.26Issue(2)：195-199,5.
玉米叶片胚性愈伤组织诱导及其与内源IAA和ABA关系的初步研究
Embryogenic Callus Induction of Maize Leaves and Related to Endogenous IAA and ABA
张东向 1张崇浩 2李杰芬2
作者信息
- 1. 齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔,161006
- 2. 北京师范大学生命科学学院,北京,100875
- 折叠
摘要
Abstract
The callus formation ability of maize leaf segments decreased gradually as their positions from basal to middle and upper segments. It corresponded to the change of endogenous IAA and ABA. The callus formations of basal segments had no associated with either 2, 4-D or combinations of 2, 4-D and BA in media, and coincided with the Logistic curve. The BA alone had no effect on callus formation. The combination of 2 mg·L-1 2, 4-D and 0.5 mg·L-1 BA was recommendable for inducing the embryogenic callus. Endogenous levels of IAA in callus cultures reduced as the culture passed and the 2, 4-D concentration increased. Endogenous levels of ABA were enhanced by 2, 4-D, especially in 2 mg·L-1. As the endogenous level of ABA in the cultures reached to maximum value, the embryogenic calli formed. Therefore the occurrence of embryogenic callus might be relate to endogenous levels of ABA, which was affected by 2, 4-D.关键词
玉米幼叶/胚性愈伤组织/2,4-D/IAA/ABAKey words
Maize leaf/Embryogenic callus/2, 4-D/IAA/ABA分类
农业科技引用本文复制引用
张东向,张崇浩,李杰芬..玉米叶片胚性愈伤组织诱导及其与内源IAA和ABA关系的初步研究[J].作物学报,2000,26(2):195-199,5.
